Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: KEGG:D06457 (HCG)
 2,659 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Indomethacin decreased Prostaglandin F 2 alpha levels in the ovaries of marmoset monkeys which were treated with PMS/HCG. The production of estradiol and progesterone, however, was unaffected by the blockade in prostaglandin synthesis. The number of ovulations following PMS/HCG treatment was significantly reduced by indomethacin treatment. These observations suggest that prostaglandins are required for follicular rupture but not for progesterone and estradiol production by the ovary.
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PMID:Effect of indomethacin on prostaglandin and steroid synthesis by the marmoset ovary in vivo. 4 Sep 10

Although many researchers have reported that RNA synthesis in the ovary is enhanced by gonadotropin treatment, there are only a few papers concerning the character of newly synthesized RNA after gonadotropin treatment. In this paper, the RNA synthesized in the ovary of immature rats after HCG treatment was qualitatively studied. Immature female Sprague-Dawley rats were administered with 0.3 mc per rat of 3H-uridine at a certain time interval after injection of HCG (10 iu/rat) and the ovaries were subsequently isolated after 15, 30 or 60 minutes. RNA was extracted from the homogenate of the ovaries according to the hot phenol method after Scherrer and Darnell. The 3H-RNA thus extracted was treated with electrophoretically purified DNase to break down and remove DNA that mingled with it. The RNA solution ultimately obtained was analysed on a 3-20% sucrose gradient. The different fractions thus separated were then subjected to measurement of radioactivity and optical density at 260 mmug. The RNA extracted from the ovary of immature untreated rat labeled with 3H-uridine for 15 minutes showed a flat pattern of radioactivity from the top to the bottom fractions with low radioactivity. Otherwise, when labeled for one hour, the RNA showed a pattern of radioactivity like those of optical density at 260 mumu with peaks of r-RNAs and t-RNA. When the ovary was pulse-labeled with 3H-uridine for 15 minutes starting 2 hours after injection of HCG, the RNA with a large S value was synthesized and the pattern of variation in radioactivity was that of rising near the bottom fraction and declining with access to the top fraction. The results obtained by labeling for 15 minutes starting 40 hours after PMS administration were similar to those obtained in immature untreated rats. The patterns of radioactivity in RNA obtained by the labeling for 15 minutes starting 2 hours after HCG and 42 hours after PMS were similar to those starting 2 hours after only HCG injection. The patterns of radioactivity became similar to those of optical density at 260 mmu, when the ovaries were labeled for 30 or 60 minutes. From these results, it was suggested that the newly synthesized RNA 2 hours after HCG was constructed from m-RMA with rapid turn over and precursors of r-RNAs and t-RNA. This RNA synthesis was blocked by pretreatment with actinomycin but not by cycloheximide. From these results, it was suggested that enhancement in RNA polymerase activity or change in template capacity of DNA which would have an effect on RNA synthesis was not based on newly synthesized protein.
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PMID:[Studies on the RNA synthesized in the ovary of immature rats after HCG administration (author's transl)]. 5 Sep 55

Aged (12- to 14-month-old) estrous and diestrous C57BL mice exhibited lower histochemically demonstrable ovarian delta5-3beta-hydroxysteroid dehydrogenase (3beta-HSD) activity in thecal, luteal, and interstitial cells, and lower (P less than 0.01) ovarian 3beta-HSD concentration and total content than did young (3-month-old) estrous animals. Administration of pregnant mare serum (PMS, 10 IU subcutaneously), followed in 40 hours by human chorionic gonadotropin (HCG, 5 IU subcutaneously) or HCG (2 IU daily for 4 days) alone, restored luteal and interstitial 3beta-HSD in aged mice. Follicular, lutea, and interstitial 3beta-HSD activity was increased in aged mice by a single PMS injection (10 IU). The total ovarian dehydrogenase concentration was increased 100% in aged animals by PMS and/or HCG administration. Restoration of histochemically demonstrable ovarian 3beta-HSD and total enzyme content in aged mice by PMS and/or HCG indicates ovarian sensitivity to gonadotropin and subnormal tropic hormone stimulation of the ovary in situ.
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PMID:Pregnant mare serum and human chorionic gonadotropin stimulate ovarian delta5-3beta-hydroxysteroid dehydrogenase in aged mice. 55 85

64 prepuberal gilts in 4 groups were treated with 400 PMS + 200 HCG (Suigonan - Vemie) (I), 100 FSH + 100 HCG) (II), 200 FSH + 200 HCG (III) ("international units") or served as untreated controls (IV). One halve of the groups laparotomized at the 12th day p.i. had ovulated 100, 25, 75 and 12,5% resp., the other halve slaughtered at the 36th day p.i. had ovulated to 83, 43, 88 and 50% resp. Sexual cycle was registered in 50, 29, 43 and 12.5% of the animals. Zystic ovaries (greater than or equal to 11 mm) developed only in a few cases in I and III.
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PMID:[Attempts at biotechnical induction of puberty in female young pigs. 1. Estrus and ovulation induction with Suigonan (Vemie) or FSH plus HCG mixtures in animals at the age of approximately 200 days]. 56 51

A biological engineering approach to induce puberty in 125 young female fattening pigs aged 190 days was undertaken on the basis of a mixture of 500 IU PMS (Prolosanserum, Dessau) with 250 IU HCG (Gonabion, Dresden). The injections were made subcutaneously. Pronounced oestrus symptoms were recorded from the external genital organs of 80% of the probands up to ten days after injection, associated with toleration in 52.8% of them. Toleration usually started on the fourth to sixth days after injection. Cycles began to develop in 57.1% up to the next oestrus period. Animals with -/x weight increase per die of 400 g exhibited lower responses. Results in terms of heat and ovulation were lower along with shorter intervals, when oestric synchronisation was undertaken 53, 32, and 17 days after the induction of puberty (20 days Suisynchron, Bernburg; 750 IU PMS).
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PMID:[Attempts at biotechnical induction of puberty in young female pigs. 2. Effects of various time intervals between one puberty induction with PMS and HCG to the following estrus synchronization on estrus and ovulation in animals about 190 days old]. 56 52

Different frequencies and periods of insemination were checked with the view to optimising puberty induction to young sows, for which purpose 500 IU PMS/250 IU HCG were injected to 571 animals in seven experiments. Insemination was carried out by deadline, since follicle stimulation was exhibited by almost all animals following the above treatment, while only some of them displayed tolerance. Advantage was taken of the induced oestrus, since its ovulation rates were higher than those associated with subsequent heat cycles, and because a synchronised second oestrus was recordable only from some of those animals which exhibited follicle stimulation in response to puberty induction. The most favourable results in terms of pregnancy and number of embryoes, against the background of the great variability in ovulation onset, were obtained, if insemination was applied 72, 96, and 120 hours after gonadotrophin treatment. From among the variants with two inseminations, good results were obtained from those 96 and 120 hours after PMS/HCG treatment.
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PMID:[Studies on the success of insemination after various insemination dates in biotechnical puberty induction of young sows]. 56 53

Three independent experiments were conducted with cyclic gilts kept under different conditions (2 x 12 on platforms and 100 from production unit), i.e. hormonally unaffected or under the usual conditions of synchronised oestrus (100 mg Suisynchron per animal and die over 20 days followed by 1,000 I.U. PMS and again followed, three days later, by 250 I.U. HCG). Blood samples were repeatedly drawn from the jugular vein and brachiocephalic vein during phases of treatment and oestrus. Acid and alkaline phosphatases were determined immediately in the plasms, and the results were calculated by variance analysis. Synchronisation, using Suisynchron, proved conducive to lowering with significance (P less than 0.01) the levels of acid and alkaline phosphatases. All changes in phosphatase values were not in full conformity within the experiments during the other phases of treatment. Phosphatase levels were not affected by discontinuation of Suisynchron nor by administration of Gn hormone. The levels of acid and alkaline phosphatases in biotechnically treated animals appeared to be lower than those in animals with spontaneous oestrus at the time of full oestrus. While the findings were of period-specific significance (P less than 0.01), the results obtained from the individual animal failed to provide specific information, since gaps were too wide between animals and between animals, on the one hand, and periods, on the other (P less than 0.01).
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PMID:[Effect of biotechnical management procedures on blood parameters in gilts. 1. Acid and alkaline phosphatase]. 56 74

Two groups of twelve gilts each kept on platforms were synchronised in two passes over 20 days, using 100 mg Suisynchron per animal and die followed by PMS treatment (1,000 I.U. Intergonan) and HCG treatment (250 I.U.). Complement fixation occurred five days after the first hormone application and was followed twelve to 15 days later by another phase of treatment, using Gravigonan (250 I.U. follicle-stimulating hormone (FSH), 500 I.U. HCG, 1 mg oestradiol benzoate in 10 ml serum of swine). Blood samples were continuously drawn during the various phases of treatment and cycle from the above animals as well as from 20 gilts synchronised in the above way and from another 20 untreated gilts. Protein-fixed iodine and copper levels were assessed from those samples. The protein-fixed iodine and copper levels of the blood were significantly reduced (P less than 0.01) by synchronisation, using Suisynchron. None of the two parameters was particularly affected by PMS and HCG treatments. Blood-borne protein-fixed iodine and copper was increased by administration of gonabione. Both parameters went up further during full oestrus at which date they were higher in untreated animals than in synchronised. (Protein-fixed iodine: 3.50/226micrograms/100 ml; P less than 0.01; copper: 0.283/0.234 mg/100 ml; P less than 0.01).
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PMID:[Effects of biotechnical management procedures on blood parameters in gilts. 2. Protein-bound iodine and copper]. 56 75

A conclusion derived from the slaughter of 69 gilts was that no role was played by the time intervals tested between puberty induction, using 500 IU of PMS and 250 IU HCG, and subsequent action to stimulate ovulation. Very good follicle maturation and follicle formation as well as the usual uterus and ovary weights were observed, no matter whether 500 IU of HCG were injected to stimulate ovulation 54, 72 or 78 hours after puberty had been induced. Ovulation was very efficiently synchronised by 500 IU of HCG in all three groups in which the ovulation figures relative to follicle formation 120 hours from puberty induction were 92.6, 94.6 or 92.7 per cent.
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PMID:[Effect of various injection times in ovulation stimulation in gilts following previous biotechnical puberty induction]. 57 Dec 66

Conversion in vitro of pregnenolone to progesterone by the ovaries from immature rats after treatment of PMS and HCG was inhibited by addition of PGE2 (1.4 X 10(-7)M) or PGF2 alpha (1.4 X 10(-7)M). Result of conversion in vitro of pregnenolone to progesterone and estradiol-17 beta by ovary of adult rat in estrus showed that the progesterone biosynthesis in the ovary was inhibited by PGF2 alpha (1.4 X 10(-7)M) but the releasing rate of progesterone from the ovary into the medium increased by about 1.25 fold. Progesterone in the medium decreased dramatically following incubation. Estradiol-17 beta in the ovarian tissue and in the medium did not differ from the control rate with addition of PGF2 alpha. When the effect of PGF2 alpha (1.4 X 10(-7)M) in vitro on the conversion of testosterone to estrone and estradiol-17 beta by the ovary from adult rat in estrus was studied, we found that the releasing rate of estrone from the ovary into the medium was increased by addition of PGF2 alpha; the rate was significantly different from control level after addition of PGF2 alpha 30 min of incubation (p less than 0.01). Thus a minute amount of PGE2 and PGF2 alpha influences steroid biosynthesis in the rat ovary.
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PMID:[Effect of prostaglandin E2 and F2 alpha on steroid biosynthesis in rat ovary (author's transl)]. 57 92


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