Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: KEGG:D03434 (Cellulase)
 512 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Spores of Chaetomium cellulolyticum were treated with 200 micrograms/ml of N-methyl-N'-nitro-N-nitrosoguanidine and seven mutants producing clear zones around their colonies on modified Vogels medium were isolated. Mutant NG7 showed altered morphological characteristics and produced more cellulases (CMCase--15 units, FPA--6.5 units, CDA--0.80 units and cellobiase--4.7 units/ml) than its parental strain (CMCase--10 units, FPA--4.5 units, CDA--0.36 units and cellobiase--2.7 units/ml). Cellulase preparation was used to saccharify rice straw, wheat straw, bagasse and sawdust, pretreated with 1% sodium hydroxide.
Acta Microbiol Pol 1985
PMID:Isolation of cellulolytic mutants of thermotolerant fungus Chaetomium cellulolyticum ATCC 32319. 242 41

This study was aimed to evaluate the pattern of cellulase biosynthesis from Aspergillusfumigatus ABK9 under submerged fermentation. Production was increased concomitantly with fungal growth up to 72 h and reached maximum (Xmax -6.72 g/l) with specific growth rate (mu max) of 0.126/h. Highest specific rate of enzyme production (q ) was found at initial medium pH of 5.0 and incubation temperature of 30 degrees C. At the same time, in the presence of 2-deoxy-D-glucose concentration of 0.5 mg/ml, the production of cellulolytic enzymes, viz, carboxymethyl cellulase activity (CMCase), filter paper degrading activity (FPase) and P-glucosidase activity reached maximum of 132.2, 21.3 and 28.9 U/ml, respectively. Cellulase biosynthesis was induced in respect to higher volumetric production rate (Qp), specific rate of enzymes production (qp, U/g biomass/h) and enzyme/biomass yield (YE/X) when grown in carboxymethyl cellulose in comparison to other saccharides as sole carbon source. Induction ratios (IR) of cellulases were between 12.3 and 24.4 in the presence of 1.5% (w/v) CMC in the culture media. The strain was quite resistant to catabolic repression by glucose up to 0.4% (w/v). Cellulases production was greatly influenced in the presence of yeast extract and potassium dihydrogen phosphate (KH2POA) as nitrogen and phosphate sources in the culture media. C/N ratio of 10.0 and C/P ratio of 4.0 proved to be the best for the production of enzyme cocktail. Along with the high production yield, the crude enzymes showed a promising cellulose hydrolyzing efficiency of rice straw, indicating the enzyme could be beneficial for its large scale industrial exploitation.
Pol J Microbiol 2013
PMID:Study on regulation of growth and biosynthesis of cellulolytic enzymes from newly isolated Aspergillus fumigatus ABK9. 2382 75

Burkholderia cepacia is an opportunistic pathogen causing infections in patients with cystic fibrosis. Patients with implanted devices are prone to B. cepacia infections due to its ability to grow as biofilms. Knowing the importance of polysaccharides in a biofilm, enzymes that degrade them were targeted as a possible candidate for antibiofilm agents. In this study, the antibiofilm potential of cellulase against B. cepacia biofilms formed on various prosthetic materials was tested. Cellulase exhibited significant antibiofilm activity against B. cepacia without having much action on its growth, thus ruling out the chance of selection pressure and subsequent development resistance.
Pol J Microbiol 2013
PMID:Cellulase inhibits Burkholderia cepacia biofilms on diverse prosthetic materials. 2445 41