Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: KEGG:D03434 (Cellulase)
 512 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We report a case of rhinitis and asthma resulting from exposure to enzymes used in an experimental plant pathology laboratory. Immediate skin-test responses were elicited to both "Cellulase" and "Macerozyme" products, and a large late onset skin reaction occurred at the Cellulase site beginning in 6 hr. Special radioallergosorbent test (RAST) substrates were prepared that showed a strong positive response to Cellulase. By the RAST method other laboratory workers were screened and another symptomatic individual was found who also showed evidence of type I hypersensitivity to both enzymes. Skin biopsy and immunodiffusion data are presented. We conclude that enzymes used to digest cell wall structures of plants are capable of eliciting both immediate and late onset skin-test reactions and positive RAST responses in susceptible persons. These reactions are thought to be mediated by IgE antibodies and can be associated with symptoms of rhinitis and asthma.
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PMID:Allergic reactions to enzymes used in plant cloning experiments. 722 29

An extracellular polysaccharide, AC-1, produced by Acetobacter polysaccharogenes is composed of beta-(1,4)glucan with branches of glucosyl residues. We found that AC-1 showed a strong activity to induce production of interleukin-12 P40 and tumor necrosis factor-alpha by macrophage cell lines in vitro. Cellulase treatment completely abolished the activity of AC-1 to induce tumor necrosis factor-alpha production by macrophages, whereas treatment of AC-1 with polymyxin B or proteinase did not affect the activity. Results of experiments using toll-like receptor (TLR) 4-deficient mice and TLR4-transfected human cell line indicated that TLR4 is involved in pattern recognition of AC-1. In vivo administration of AC-1 significantly reduced the serum levels of ovalbumin (OVA)-specific IgE and interleukin-4 production by T cells in response to OVA in mice immunized with OVA. AC-1, a soluble branched beta-(1,4)glucan may be useful in prevention and treatment of allergic disorders With IgE production.
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PMID:Soluble branched beta-(1,4)glucans from Acetobacter species show strong activities to induce interleukin-12 in vitro and inhibit T-helper 2 cellular response with immunoglobulin E production in vivo. 1279 62