Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: KEGG:D03345 (
beta-Galactosidase
)
434
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Equine herpesvirus-1 (EHV-1) latency in murine olfactory bulbs was demonstrated by a novel combined in situ PCR and in vitro protein synthesis method (in situ PS-PCR). The Escherichia coli lacZ gene replacing a deletion in EHV-1 gene 71 (EUS4) was thus amplified and transcribed/translated in situ followed by enzymatic detection using X-Gal (5-bromo-4-chloro-3-indoyl-beta-D-galactopyranoside).
beta-Galactosidase
was found to be concentrated over mitral/tufted neurons indicating those to be the sites of latency. Our results suggest that, in common with other alpha-herpesviruses, EHV-1 can establish latency in central nervous system neurons and that the unique
membrane glycoprotein
encoded by EHV-1 gene 71 is nonessential for infection of neural tissues.
...
PMID:Demonstration of equine herpesvirus-1 neuronal latency in murine olfactory bulbs using a novel combined in situ PCR and protein synthesis method. 912 71
