Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: KEGG:D03345 (beta-Galactosidase)
 434 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

It has recently been demonstrated that prostaglandins (PGs) play an important role in the ovulation, the onset of menstruation and labor pain and other reproductive phenomena. The purpose of this study is to develop the enzyme immunoassay (EIA) for PGF2 alpha to estimate the PGF2 alpha level in body fluids. beta-Galactosidase-PGF2 alpha conjugate and bovine serum albumin-PGF2 alpha conjugate were prepared by a mixed anhydride method. PGF2 alpha was extracted with ethylacetate from an acidified sample. EIA was carried out using a double antibody method. As for the conjugation ratio of PGF2 alpha and beta-Galactosidase, 5, 10, 20, 40 and 80 were examined. Recovered enzyme activity and sensitivity of the method were better in the enzyme of conjugation ratio 10 than in the other conjugation ratios, 5, 20, 40, 80. Values measured by RIA and EIA were well correlated. The correlation rate was 0.84. The recovery rate was 102.3%. The sensitivity of the standard curve was 5-100 pg/tube. PGF2 alpha in the menstrual blood of the 18 women was determined with the EIA method. The mean value for PGF2 alpha in menstrual blood is 20.9 ng/ml (S.D. = 11.4).
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PMID:[The development of enzyme immunoassay for prostaglandin F2 alpha]. 354 38

We present the observation of separated protein bands after polyacrylamide (PAA) gel electrophoresis based on the staining-free detection of their ultra violet (UV)-induced fluorescence employing deep UV confocal fluorescence microscopy. Mixtures of the three biological compounds beta-Galactosidase (from Escherichia coli), apo-Transferrin (bovine) and bovine serum albumin (BSA) have been separated and a staining free detection limit below 80 pg (7.0 x 10(8) molecules) per band has been achieved. This corresponds to approximately 270 molecules in the detection volume for confocal microscopy.
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PMID:Ultrasensitive staining-free protein detection after PAA gel electrophoresis using deep UV fluorescence. 1789 99

Magnetic resonance imaging (MRI) permits noninvasive three-dimensional imaging of opaque organisms. Gadolinium (Gd(3+)) complexes have become important imaging tools as MRI contrast agents for MRI studies, though most of them are nonspecific and report solely on anatomy. Recently, MRI contrast agents have been reported whose ability to relax water protons is triggered or greatly enhanced by recognition of a particular biomolecule. This new class of MRI contrast agents could open up the possibility of reporting on the physiological state or metabolic activity deep within living specimens. One possible strategy for this purpose is to utilize the increase in the longitudinal water proton r(1) relaxivity that occurs upon slowing the molecular rotation of a small paramagnetic complex, a phenomenon which is known as receptor-induced magnetization enhancement (RIME), by either binding to a macromolecule or polymerization of the agent itself. Here we describe the design and synthesis of a novel beta-galactosidase-activated MRI contrast agent, the Gd(3+) complex [Gd-5], by using the RIME approach. beta-Galactosidase is commonly used as a marker gene to monitor gene expression. This newly synthesized compound exhibited a 57% increase in the r(1) relaxivity in phosphate-buffered saline (PBS) with 4.5% w/v human serum albumin (HSA) in the presence of beta-galactosidase. Detailed investigations revealed that RIME is the dominant factor in this increase of the observed r(1) relaxivity, based on analysis of Gd(3+) complexes [Gd-5] and [Gd-8], which is generated from [Gd-5] by the activity of beta-galactosidase, and spectroscopic analysis of their corresponding Tb(3+) complexes, [Tb-5] and [Tb-8].
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PMID:A Gd3+-based magnetic resonance imaging contrast agent sensitive to beta-galactosidase activity utilizing a receptor-induced magnetization enhancement (RIME) phenomenon. 1799 79