Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: KEGG:D03301 (PDL)
 658 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Background: Fibrin and cytokines in platelet-rich fibrin (PRF) can be combined into a powerful biological scaffold, which is an integrated reservoir of growth factors involved in tissue regeneration. Insulin-like growth factor-1 (IGF-1) is a kind of effective mitogenic protein, which can enhance osteogenic differentiation of periodontal ligament fibroblasts. However, whether PRF and IGF-1 can stimulate the osteogenic differentiation and osteogenesis of human periodontal ligament stem cells (PDLSCs) remains unclear. This study aims to investigate the osteogenic capability of PDLSCs in vitro and in vivo after being separated from human PDL tissues, purified with STRO-1 and treated with PRF and IGF-1. Methods: The proliferative capabilities of PDLSCs under different conditions were analyzed via methyl thiazolyl tetrazolium (MTT), growth curve, alkaline phosphatase activity, reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting, respectively. Results: PRF and IGF-1 significantly promoted the growth, proliferation and differentiation of PDLSCs, up regulated the expressions of Runt-related transcription factor 2 (RUNX2), osterix (OSX) and osteocalcin (OCN), phosphorylated extracellular signal-regulated kinase (ERK) and phosphorylated c-Jun N-terminal kinase (JNK) in stem cells. Conclusion: Our data indicate that PRF and IGF-1 facilitate the proliferation of alveolar osteoblast via the activation of the mitogen-activated protein kinase (MAPK) signaling pathway.
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PMID:Roles of PRF and IGF-1 in promoting alveolar osteoblast growth and proliferation and molecular mechanism. 3194 4