Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: KEGG:D03301 (PDL)
 658 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Alginate-amino acid derivatives were explored to engineer poly(DL-lactic acid)(PDL-LA) as glycocalyx-like surface to promote cell adhesion and growth. Four different kinds of alginate-amino acid derivatives were synthesized to mimic the glycocalyx of cell membrane to promote chondrogenesis. The alginate-amino acid derivatives were characterized by FT-IR, 1H NMR and UV spectra and the amino acid content on alginate-amino acid derivatives was given by ninhydrin-UV method. A new strategy, entrapment, was then employed to modify PDL-LA membranes with alginate and its amino acid derivatives. The results of XPS, ATR-FTIR and contact angle confirmed that a stable thin film of alginate and its amino acid derivatives can be entrapped on the surface of PDL-LA membrane. The chondrocyte cytocompatibility test and MTT assays indicated that the alginate-amino acid derivatives modified PDL-LA membranes could promote chondrogenesis. The novel surface treatment method may have potentials for tissue engineering and other biomedical applications.
 ...
PMID:Surface engineering of poly(DL-lactic acid) by entrapment of alginate-amino acid derivatives for promotion of chondrogenesis. 1210 85

We report the development of new biomacromolecule coatings on biodegradable biomaterials based on electrostatic assembly of extracellular matrix-like molecules. Poly(ethylene imine) (PEI) was employed to engineer poly(dl-lactide) (PDL-LA) substrate to obtain a stable positively charged surface. An extracellular matrix- (ECM-) like biomacromolecule, gelatin, was selected as the polyelectrolyte to deposit on the activated PDL-LA substrate via the electrostatic assemble technique. The extracellular matrix-like multilayer on the PDL-LA substrate was investigated by attenuated total reflection (ATR-FTIR), X-ray photoelectron spectrscopy (XPS), contact angle, and atomic force microscopy (AFM). The gradual buildup of the protein layer was investigated by UV-vis spectra, and it was further given a quantitative analysis of the protein layer on the PDL-LA substrate via the radioiodination technique. The stability of the protein layer under aqueous condition was also tested by the radiolabeling method. Chondrocyte was selected as the model system for testing the cell behavior and morphology on modified PDL-LA substrates. The chondrocyte test about cell attachment, proliferation, cell activity and cell morphology by SEM, and confocal laser scanning microscopy (CLSM) investigation on extracellular matrix-like multilayer modified PDL-LA substrate was shown to promote chondrocyte attachment and growth. Comparing conventional coating methods, polyelectrolyte multiplayers are easy and stable to prepare. It may be a good choice for the modification of 3-D scaffolds used in tissue engineering. These very flexible systems allow broad medical applications for drug delivery and tissue engineering.
 ...
PMID:Surface engineering of poly(DL-lactide) via electrostatic self-assembly of extracellular matrix-like molecules. 1262 35

The ligand-tethered poly(ethylene oxide-propylene oxide-ethylene oxide) (PEO-PPO-PEO) triblock copolymer was explored to engineer poly(DL-lactic acid) (PDL-LA) material to promote cell attachment and growth. The PEO-PPO-PEO was activated by methyl sulfonyl chloride and the amino acid, and peptide were attached. By blending the PDL-LA with the ligand-tethered PEO-PPO-PEO derivatives, the surface of modified PDL-LA film was investigated by ATR-FTIR, XPS and contact angle. The chondrocytes test on different PDL-LA films indicated that the PEO-PPO-PEO amino acid and RGD derivatives modified PDL-LA films could promote chondrocyte attachment and growth. This simple surface treatment method may have potentials for tissue engineering and other biomedical applications.
 ...
PMID:Surface tailoring of poly(DL-lactic acid) by ligand-tethered amphiphilic polymer for promoting chondrocyte attachment and growth. 1473 50