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Query: KEGG:D03063 (BCG vaccine)
 1,125 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The results of nonspecific immunotherapy with BCG vaccine in 98 cases of melanoma, breast cancer and other malignancies were used in evaluating the frequency and degree of side-effects and complications arising in cancer patients during this treatment. The procedure proved to be safe irrespective of patients' age. Prevention and treatment of side-effects such as fever, water-salt disorders, anorexia, interstitial hepatitis and promotion of tumor growth are discussed.
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PMID:[Treatment of the complications occurring in BCG vaccine immunotherapy of patients with malignant neoplasms]. 646 96

Standardisation and control of the live Mycobacterium bovis BCG (BCG) vaccine is performed as specified by the World Health Organisation (WHO) and the European Pharmacopoeia (EP). The conventional viable count for control of potency of BCG vaccine is performed by culturing on solid medium. This assay method is not only time consuming but may give variable results. A tetrazolium salt assay has been developed and evaluated as a potential additional, or replacement, test for determining number of viable organisms. The tetrazolium salts 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and 2,3-bis-(2-methoxy-4-nitro-5-sulphenyl)-(2H)-tetrazolium-5-carb oxanilide (XTT) used as alternative substrates in the assay both gave more rapid and reproducible results than the conventional viable count. XTT showed greater sensitivity than MTT with a lower detection limit of about 7x10(4) colony forming units (c.f.u.) ml(-1). The XTT assay has proven effective for determining viability of suspensions prepared from several BCG vaccine substrains, covering a range of viable units, without the need for modification. This assay is easily performed and takes just 48 h to produce an estimate of viable cell content compared with 3 weeks for the conventional method.
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PMID:Development of a tetrazolium salt assay for rapid determination of viability of BCG vaccines. 1039 24

Experiments were carried out to determine the effect of various adjuvants on the preservability of dried BCG vaccine at 37 degrees C. The substances tested included carbohydrates, proteins, amino-acid derivatives, synthetic polymers, peptides and polypeptides, and various combinations of protein, sugar, and salt.The resulting vaccines were preserved for periods of up to 8 months at 37 degrees C, and were then subjected to culture tests. The highest BCG survival-rate was obtained with sodium glutamate vaccine, no significant difference being observed between the survival-rates with l- and with dl-sodium glutamate. Of the three concentrations of sodium glutamate tested-that is, 1%, 5%, and 10%-the 1% concentration gave the highest survival-rate.From the results obtained with 1% sodium glutamate as adjuvant-a survival-rate per original suspension of about 10% and a viable-unit level of about 10 in 10(-6) mg of bacilli after 6-8 months' preservation-it may be concluded that it is possible to produce a dried BCG vaccine the viability of which will not be adversely affected by storage for several months at room temperature, even in hot climates.
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PMID:Effect of adjuvant on preservability of dried BCG vaccine at 37 degrees C. 1335 42