Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: KEGG:D02259 (NaI)
 1,823 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Polycyclic aromatic hydrocarbons, e.g., benzo[a]pyrene (B(a)P) are known carcinogens/mutagens. These compounds may be metabolized by the P450 mixed function monooxygenase to more nucleophilic compounds which may form adducts to the cellular macromolecules, e.g., DNA, RNA, and proteins. We have used synchronous fluorescence scanning for the assay of DNA adduct formation. In our earlier work with in vitro exposed human lymphocytes we estimated the adduct formation (femtomoles B(a)P per microgram DNA) to be higher than that estimated by other workers. We suggested that this difference may be related to the DNA isolation method used. In order to elucidate these differences we compared DNA adduct formation in human lymphocytes where DNA was isolated by the two different methods, i.e., using phenol extraction or the Gene Clean method. The data demonstrate that the phenol extraction procedure gives a yield of adducts per microgram DNA lower than that obtained by the Gene Clean method. The principle of the Gene Clean method for DNA isolation is protein denaturation by means of NaI followed by catching of DNA by absorption on silica particles. In contrast, the phenol extraction method is based upon phenol-mediated denaturation of proteins in the cell lysate leaving the hydrophilic nucleotides in the aqueous phase. However, during adduct formation more lipophilic adducts derived from DNA may redistribute between the aqueous phase and the phenol phase. In support of this theory we found higher adduct concentration per microgram DNA by the Gene Clean method 40 to 60 times than that found by the phenol method.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Method for estimation of benzo[a]pyrene DNA adducts. 144 82

Pochonia chlamydosporia produces radicicol (1), a potent antifungal and anticancer product. NaBr, but not NaF, NaCl or NaI, inhibited the biosynthesis of 1 in P. chlamydosporia in a dose-dependent manner, accompanied by the formation chlorine-lacking monocillins II-V (2-5), indicating that the dedicated halogenase, Rdc2 had been inhibited. RT-PCR analysis confirmed that transcription of rdc2 was selectively inhibited by Br(-), whereas the putative P450 epoxidase gene, rdc4, was not affected.
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PMID:Specific inhibition of the halogenase for radicicol biosynthesis by bromide at the transcriptional level in Pochonia chlamydosporia. 2093 51