Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: KEGG:D02259 (NaI)
 1,823 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

DNA typing of the ABO blood groups from single fingerprints was examined. Genomic DNAs were extracted from fingerprints on slide glasses by a simple method with NaI and N-lauroyl-sarcocinate, and then subjected to the PCR (Polymerase chain reaction). Two PCR fragments spanning positions 258 and 700 of the cDNA sequence of the ABO locus were amplified. Then, the PCR products were digested with a restriction enzyme, Kpn-1 or Msp-1, for each site. The digested PCR products were analyzed by electrophoresis on polyacrylamide gels for RFLP (Restriction-fragment length polymorphism). Bands on the gel were visualized by silver staining. Genotyping from single fingerprints after treatment with aluminium powder was also possible by these techniques.
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PMID:[ABO genotyping of fingerprints by the PCR-RFLP method]. 786 40

An automatic and rapid DNA typing system was employed for personal identification, using fragmentary tissue samples from victims in an airplane accident. Two victims were crushed into small pieces, and 33 samples suspected to belong to them were recovered from under the sea. From each sample, 10 mg was used for testing. The parents' bloods of two presumptive victims were also examined. DNA extraction from samples was performed by the NaI method, and the obtained DNA samples were analyzed with the ABI PRISM system. Among 33 samples, 31 samples were identified to be human tissues, possibly from two victims. The other two samples seemed to be parts of marine animals. ABO blood group, STR polymorphism, and mitochondrial DNA polymorphism typing were possible in every examined human sample. Two victims' fragmentary tissues were identified by determining ABO genotype, STR type and mitochondrial DNA type. The system we employed enabled an accurate typing of many fragmentary samples in a short time, thus contributing to the fast and secure identification of many victims in such cases as big air accidents.
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PMID:The personal identification of many samples recovered from under the sea. 1097 5