Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: KEGG:D02011 (FAD)
 5,530 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

By affinity chromatography, germins G and psiG were purified from roots of wheat seedling and wheat embryos imbibition for 4 h. Characterization of the germins G and psiG were studied. The results showed that G and psiG were stable at temperatures lower than 60 degrees C, and had an optimum pH at 3.5. The Km value of G for oxalate was 0.084 mmol/L, while that of psiG was 0.053 mmol/L. Oxalate showed substrate inhibition effect above 0.2 mmol/L on G and psiG. EDTA, NH(+)(4), Mn(2+), Mg(2+), Na(+) and K(+) at a concentration of 0.1 mmol/L had no effect on OxO activity. CO(2-)(3), NO(-)(3) and SO(2-)(4) at a concentration of 0.1 mmol/L inhibited partially the activities of G and psiG, 0.1 mmol/L Cu(2+), Fe(2+), Al(3+) completely inhibited the activities of G and psiG, but addition of 0.1 mmol/L EDTA partially relieved the inhibition, which indicated that metal cations may make the oxalate unavailable by chelating it. H(2)PO(-)(4) and HPO(2-)(4) inhibited the activity of G, but had no effect on psiG. Riboflavin, FMN and FAD inhibited psiG, but FMN and FAD had no effect on G.
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PMID:[Purification and some characteristics of germins G and psiG from wheat]. 1562 87

p-Hydroxybenzoate hydroxylase (PHBH) is an FAD-dependent monooxygenase that catalyzes the hydroxylation of p-hydroxybenzoate (pOHB) to 3,4-dihydroxybenzoate in an NADPH-dependent reaction and plays an important role in the biodegradation of aromatic compounds. PHBH from Corynebacterium glutamicum was crystallized using the hanging-drop vapour-diffusion method in the presence of NaH(2)PO(4) and K(2)HPO(4) as precipitants. X-ray diffraction data were collected to a maximum resolution of 2.5 A on a synchrotron beamline. The crystal belongs to the hexagonal space group P6(3)22, with unit-cell parameters a = b = 94.72, c = 359.68 A, gamma = 120 degrees . The asymmetric unit contains two molecules, corresponding to a packing density of 2.65 A(3) Da(-1). The structure was solved by molecular replacement. Structure refinement is in progress.
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PMID:Expression, purification, crystallization and initial crystallographic characterization of the p-hydroxybenzoate hydroxylase from Corynebacterium glutamicum. 1800 46

Redox processes in nano-TiO(2)-flavin adenine dinucleotide (TiO(2)-FAD) layer-by-layer assembled films on ITO substrate electrodes are investigated and compared in contact to aqueous electrolyte media (for dilute and saturated electrolyte) and in contact to solid humidified salt electrolyte (for extreme salt levels and different types of salts). Under these unusual conditions an aqueous microphase present at the gas | salt | electrode interface allows voltammograms to be obtained and redox processes to be analysed. It is demonstrated that the 2-electron 2-proton reduction of FAD can be used as reporter redox system to determine local pH at the electrode | gas | salt interface as pH 15, 12, 7 for contacts to K(3)PO(4), K(2)HPO(4), and KH(2)PO(4), respectively. Exposure to gases such as carbon dioxide is shown to lead to unexpected changes in surface pH. In the future, bio-electrochemical microphase processes under halophilic conditions could be useful for air-quality and rapid gas sensing devices.
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PMID:Nano-TiO(2)-flavin adenine dinucleotide film redox processes in contact to humidified gas | salt electrolyte. 2238 3