Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
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Drug
Enzyme
Compound
Query: KEGG:D02011 (
FAD
)
5,530
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
We previously demonstrated the efficient production of trans 10, cis 12-conjugated linoleic acid (t10c12-CLA) in
Lactococcus lactis
by ectopically expressing a
Propionibacterium acnes
isomerase (pai) gene and also mentioned that a recombinant strain was unable to accumulate t10c12-CLA product, despite the normal transcription. Here, the molecular analysis indicated that this mutated strain harbors a
pai
gene with a single-nucleotide mutation converting GC
50
A to
GTA
, leading to a corresponding change of Alanine residue into Valine. The expression of the reverse mutation resulted in the recovery for enzyme activity. Site-directed mutagenesis indicated that the codon usage of Val
17
was not responsible for the enzyme inactivation in the Ala
17
Val mutation. Western blot analysis revealed that the recombinant PAI protein was not detectable in the His tag-marked Ala
17
Val mutant. It is, therefore, reasonable to assume that Ala
17
residue is critical for PAI functionality.
Abbreviations:
pai:
propionibacterium acnes
isomerase; CLA: conjugated linoleic acid; t10c12-CLA: trans 10, cis 12-CLA; LA: linoleic acid (18:2n-6);
FAD
: flavin adenine dinucleotide.
...
PMID:A single amino acid substitution in the FAD-binding domain causes the inactivation of
Propionibacterium Acnes
isomerase. 3188 76
