Gene/Protein
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Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
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Target Concepts:
Gene/Protein
Disease
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Query: KEGG:D02011 (
FAD
)
5,530
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Marine-sourced actinomycete genus
Streptomyces
continues to be an important source of new natural products. Here we report the complete genome sequence of deep-sea-derived
Streptomyces olivaceus
SCSIO T05, harboring 37 putative biosynthetic gene clusters (BGCs). A cryptic BGC for type I polyketides was activated by metabolic engineering methods, enabling the discovery of a known compound, lobophorin CR4 (
1
). Genome mining yielded a putative lobophorin BGC (
lbp
) that missed the functional
FAD
-dependent oxidoreductase to generate the d-kijanose, leading to the production of lobophorin CR4 without the attachment of d-kijanose to C17-OH. Using the gene-disruption method, we confirmed that the
lbp
BGC accounts for lobophorin biosynthesis. We conclude that metabolic engineering and genome mining provide an effective approach to activate cryptic BGCs.
...
PMID:Genome Sequencing of
Streptomyces olivaceus
SCSIO T05 and Activated Production of Lobophorin CR4 via Metabolic Engineering and Genome Mining. 3163 59
