Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: KEGG:D02003 (NBT)
 1,323 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In order to clarify the frequency of p16INK4 gene inactivation and its relationship with retinoblastoma (Rb) gene expression in non-small cell lung cancer, in situ double hybridization was established with Bio-labelled Rb cDNA probe and Dig-lablled p16INK4 cDNA probe, and immunohistochemistry was carried out with polyclone rabbit antibodies to human p16INK4 and Rb protein. The results showed that positive hybridization of p16INK4 mRNA showed blue with the detecting system of alkiline phosphatase NBT/BCIP, positive hybridization of Rb mRNA showed red with the detecting system of HPR-AEC. The negative ratios of p16INK4 and Rb gene transcription were 22.6%(7/31) and 16.1%(5/31). The results of immunohistochemistry showed that the negative ratio of p16INK4 protein was 42%(13/31) with p16INK4 polyclone antibody, the negative ratio of pRb was 19.4%(6/31) with polyclone antibody. The data indicated that p16INK4 gene and Rb gene are synergistic in the progression of lung cancer, p16INK4 gene plays a significant role in the development of non-small cell lung cancer.
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PMID:[Synergistic effect of the expression of p16INK4 gene and retinoblastoma gene in lung cancer]. 1148 Jan 70

Aims. In this work we studied cytodifferentiation effects of newly characterized prenyl flavonoid 4'-O-methylkuwanon E (4ME) isolated from white mulberry (Morus alba L.). Main Methods. Cell growth and viability were measured by dye exclusion assay; cell cycle and surface antigen CD11b were monitored by flow cytometry. For the cytodifferentiation of cells the NBT reduction assay was employed. Regulatory proteins were assessed by western blotting. Key Findings. 4ME induced dose-dependent growth inhibition of THP-1 cells, which was not accompanied by toxic effect. Inhibition of cells proliferation caused by 4ME was associated with the accumulation in G1 phase and with downregulation of hyperphosphorylated pRb. Treatment with 4ME led to significant induction of NBT-reducing activity of PMA stimulated THP-1 cells and upregulation expression of differentiation-associated surface antigen CD11b. Our results suggest that monocytic differentiation induced by 4ME is connected with up-regulation of p38 kinase activity. Significance. Our study provides the first evidence that 4ME induces the differentiation of THP-1 human monocytic leukemia cells and thus is a potential cytodifferentiating anticancer agent.
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PMID:Flavonoid 4'-O-Methylkuwanon E from Morus alba Induces the Differentiation of THP-1 Human Leukemia Cells. 2573 34