Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: KEGG:D02003 (NBT)
 1,323 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The effect of PHA-activated mononuclear-cell (MN) supernatants on various polymorphonuclear-leucocyte (PMN) functions were assessed. Treatment of PMN with PHA-activated MN-cell supernatants resulted in greater electrophoretic mobility, indicating an increase in the negative surface charge. PMN directional motility was inhibited in the presence of active supernatants but was not affected by a pulse exposure of the PMN to these supernatants. Neither control nor active supernatants were chemotactic for PMN, but treatment of these cells with active supernatants produced an increase in their phagocytic activity, their ability to reduce NBT and in their glucose oxidation through the hexosemonophosphate shunt. Bactericidal capacity of these PMN was unaltered. Specific loss of leucocyte inhibitory factor (LIF) activity from supernatants of PHA-activated MN cells followed their absorption with PMN cells but not with human MN cells or guinea-pig peritoneal exudate cells. Furthermore, acquired inhibition of migration of the absorbing PMN was observed.
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PMID:The effect of PHA-activated MN-cell supernatants on polymorphonuclear leucocyte function. 58 67

Immune status of 22 patients of ataxia telangiectasia was studied over a period of 8 yr (mean age of patients: 9.5 +/- 3 yr; 9 of 22 were siblings). Low T-cell number was observed in 14 of 19 patients but the response to PHA challenge done in 10 patients was normal and migration inhibition to BCG antigen was positive in 6 of 6 patients. IgM defect was seen in 2 out of 18 patients and serum IgA was deficient in 10 out of 18 patients. Salivary IgA was also absent in these children. Four children had high spontaneous NBT reduction. None of the patients had lymphoma, leukemia or any other malignancy at the time of presentation. Candida killing was normal in all patients. The presenting feature related to the CNS in almost all children and gross infections were not seen.
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PMID:Immune status in ataxia telangiectasia. 193 11

Immunological status of children after thymectomy performed at the age between 2 months and 15 years for teratoma in 5, and hyperplasia in 1. Cellular immunity parameters--as percent and total T lymphocyte count and their PHA reactivity, humoral immunity--as IgG, IgA, IgM concentrations, and phagocytosis--as phagocytosis index, NBT test, complement components--C3c, C4--concentration were evaluated between 6 and 12 years following surgery. No abnormalities within investigated parameters, nor the history of more frequent infections or other immunological disorder were detected.
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PMID:[Evaluation of immune response after thymectomy]. 215 15

The effect of piroxicam therapy (20 mg/day for 15 days) on various polymorphonuclear granulocyte (PMN) responses and on PMN elastase concentration was investigated in nine patients with active rheumatoid arthritis. Peripheral blood and synovial fluid samples were collected before starting therapy and 12 h after the last dose of the drug. All patients were evaluable for peripheral blood analysis and six for synovial fluid analysis. Piroxicam therapy had no effect on PMN random migration and phagocytosis, while it significantly reduced both FMLP-induced aggregation and FMLP-induced chemotaxis. This seems mainly due to an effect on FMLP binding, as no differences were observed after therapy in PMA- and PHA-induced aggregation as well as in serum-induced chemotaxis. In contrast, a marked impairment of NBT test and PMA- and FMLP-induced superoxide anion (O2-) production was found after piroxicam therapy. This effect was as evident in peripheral blood as in synovial fluid PMN. Also, a significant reduction in synovial fluid PMN number and synovial fluid PMN elastase concentration (elastase-alpha 1-proteinase complex) was found after treatment. It is concluded that piroxicam may act at different sites on various PMN responses. Its effect on O2- generation and PMN elastase concentration in synovial fluid may have an important role in reducing destruction of arthritic joint tissue.
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PMID:Effect of piroxicam therapy on granulocyte function and granulocyte elastase concentration in peripheral blood and synovial fluid of rheumatoid arthritis patients. 254 13

Cord blood T cells, purified by nylon wool column, were incubated with PHA for 18 hours, and the supernatant were harvested as PHA-TCM. The results demonstrated that PHA-TCM could significantly inhibit the growth of U937 cells and reduce their 3H-TdR incorporation and spontaneous colony formation. After 3-6 days of treatment with PHA-TCM in culture, part of U937 cells became macrophage-like, latex-phagocytic, and capable of reducing NBT dye. On the contrary, HL-60 cells did not respond to PHA-TCM inhibition, indicating the selective effect of PHA-TCM.
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PMID:[Effect of PHA-T cell conditioned medium (PHA-TCM) on leukemic cells]. 269 Mar 52

The immune systems were compared in 23 subjects with isolated IgA deficiency and 15 controls with normal levels of IgA. The IgA deficient group had higher levels of serum IgM, and lower stimulation indices for the mitogens Con A, PWM and PHA; only the index for PHA was statistically significant. Their suppressor T-cell activity was decreased and chemotactic activity significantly decreased. They also exhibited a decrease in Leu 3, and a significantly lower ratio of Leu 3 to Leu 2. The mean percent of positive NBT neutrophils was decreased. An imbalance in the immunoregulatory mechanism is suggested as a possible explanation for IgA deficiency.
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PMID:The immune system in isolated IgA deficiency. 387 11

T-cells were investigated by the E rosette assay, PHA stimulation, allogeneic MLC and CML responses, in a group of 100 healthy aged people over 70 years old, and compared to young control individuals less than 45 years old. No major difference was found between the two groups, except for PHA stimulation which was significantly reduced in old individuals when low mitogen concentration was used. These observations suggest that only some T cells subsets are affected by ageing, without modification of the total T lymphocyte number. Contrarily, the phagocytic activities of polymorphonuclear cells assessed in a group of 38 aged people were dramatically decreased, using either the NBT test or a direct phagocytosis assay.
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PMID:Immunological studies in human ageing. I. In vitro functions of T cells and polymorphs. 645 27

The clinical and laboratory features of a child with chronic granulomatous disease (CGD) and IgA deficiency and his family are presented. Bactericidal and NBT dye reduction studies confirmed the diagnosis of CGD in the patient and the carrier state in the mother. No other family member had IgA deficiency. The manifestations of the IgA deficiency include multiple autoimmune antibodies, progressive pulmonary dysfunction but no gastrointestinal or rheumatoid symptoms. The etiology of the IgA deficiency appears to be a failure in terminal B cell differentiation as evidenced by the presence of normal numbers of IgA bearing cells detected by a fluorescent monospecific antisera, a normal profile of T cell subpopulations, normal responses to the mitogens PHA, Con A, PWM, and antigens C. albicans, E. coli, and S. aureus, and the absence of suppressor cell activity in co-culture assays. The significance of the association of these two disorders is discussed.
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PMID:Chronic granulomatous disease and selective IgA deficiency. 698 58

The effects are reported of a combination therapy of i.v. C. parvum and cyclophosphamide on the survival time and immune responses of patients with inoperable squamous-cell carcinoma of the bronchus. The immune status of the patients was evaluated by determining the antibody response to C. parvum, the E and EAC rosettes, the PHA response of blood lymphocytes, the skin-test reactivity to Candida and PPD, the response to DNCB and the chemotaxis and NBT-dye reduction capacity of neutrophil leucocytes. The survival time of patients treated with the combination therapy was found to be significantly shorter than that of untreated patients and of those receiving cyclophosphamide only. Severe side effects were observed after C. parvum infusions, with no decrease on repeated administration. The effect of C. parvum on the different immune parameters of cyclophosphamide-treated patients was negligible, though there was a normal antibody response to C. parvum.
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PMID:Harmful effects of i.v. Corynebacterium Parvum given at the same time as cyclophosphamide in patients with squamous-cell carcinoma of the bronchus. 738 58

Immunologic and cytochemical studies were done in 162 patients with myeloid forms of acute leukosis, among whom 130 had acute myeloblastic leukemia (AML), and thirty two--and acute myelomonoblastic leukemia (AMML), before and after the therapy instituted. Immunocorrection was attempted with immunomodulating agents prodigiosane and tactivin. Striking inhibition of T-cellular immunity (T-total, T-active, TPhres, TPhsen, as well as blast transformation of lymphocytes with PHA) was revealed. All patients experienced significant rise in circulating immune complexes before treatment. Investigation designed to study intracellular metabolism of neutrophils demonstrated significant decline in the activities of G-6-P-ase, NAD- and NADP-oxidases, cationic proteins and NBT-test. Immunocorrective agents improve the state of immunologic reactivity, make for arresting infections and inflammatory processes and prolongation of remission in patients with acute myeloid forms of leukoses.
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PMID:[Immunological deficiency in patients with the myeloid forms of acute leukemia and the methods for its correction]. 760 94


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