Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: KEGG:D02003 (NBT)
 1,323 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Report on a boy, now 3 years old, who presented the typical clinical picture of a BCG generalization during infancy. The BCG agents were verified in the lymph node. Intensive tuberculostatic therapy in combination with the transfer factor failed. The negative NBT test revealed a progressive septic granulomatosis.
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PMID:[Clinical picture of a generalized BCG vaccination tuberculosis as an expression of a progressive septic granulomatosis (author's transl)]. 102 Mar 60

NBT test basal values and those after cell stimulation with killed BCG culture were measured by spectrophotometry in alveolar macrophages isolated from bronchoalveolar lavage fluid, in peripheral blood neutrophils and monocytes of 49 patients with newly detected pulmonary tuberculosis. Stimulation coefficient was estimated as the ratio of induced to spontaneous NBT-test values. The findings evidence that spontaneous NBT-test values are regularly growing in all three cell types, and the stimulation coefficients lowers as the process grows in severity at the expense of pulmonary tissue disintegration and bronchial obstruction; these parameters adequately reflect the status of the body specific reactivity. Significant correlations were revealed between spontaneous NBT-test parameters in all 3 cell types and their stimulation coefficients. This permits a conclusion that spontaneous and induced NBT-test of the peripheral blood cells may help assess alveolar macrophage function.
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PMID:[Evaluation of the functional activity of alveolar macrophages based on the results of a study of blood cells]. 170 82

Immune status of 22 patients of ataxia telangiectasia was studied over a period of 8 yr (mean age of patients: 9.5 +/- 3 yr; 9 of 22 were siblings). Low T-cell number was observed in 14 of 19 patients but the response to PHA challenge done in 10 patients was normal and migration inhibition to BCG antigen was positive in 6 of 6 patients. IgM defect was seen in 2 out of 18 patients and serum IgA was deficient in 10 out of 18 patients. Salivary IgA was also absent in these children. Four children had high spontaneous NBT reduction. None of the patients had lymphoma, leukemia or any other malignancy at the time of presentation. Candida killing was normal in all patients. The presenting feature related to the CNS in almost all children and gross infections were not seen.
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PMID:Immune status in ataxia telangiectasia. 193 11

67 patients with mammary carcinoma were submitted to an immunological control examination. This control comprised 14 tests which determined the efficacy of the immune systems, among others: activity of complement, properdin, lysozyme, and beta-lysin, the rate of immunoglobulins, and the behavior of leucergy, rosette tests, NBT, BLT, and tuberculin test. The thoroughly executed examinations did not show any significant difference between patients with active neoplasms and patients in remission stage. The analysis of the results achieved for patients in an advanced stage (TNM) only showed a significant increase (p less than 0.05) of IgG in stage III/IV. The patients were treated with CVF, levamisole (Decaris), and BCG. The observation period was twelve months. The results achieved were discussed in detail.
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PMID:[Evaluation of the efficiency of the immune system after immunostimulation in patients with breast cancer]. 641 41

The capacity of macrophages activated in vivo and in vitro to kill Plasmodium yoelii was investigated. Macrophages activated by BCG-, Con A-, or malaria-induced lymphokines (LK) were cultured with P. yoelii-parasitized erythrocytes (PE). In some experiments, effector and target cells were separated by a 0.45-micron filter. Parasite viability was assessed a) in vivo by injection of mice and quantitative detection of parasites by RIA or b) in vitro by the incorporation of 3H amino acids into parasite proteins. Activated macrophages killed target PE in a dose-dependent manner by elaborating a membrane-permeable soluble factor(s). The addition of small amounts of immune serum augmented the killing of the parasites. LK-activated macrophages underwent an oxidative burst upon the phagocytosis of PE as evidenced by the accumulation of reduced formazan in the NBT assay. The magnitude of the oxidative response corresponded to the number of parasites that were ingested. The phagocytosis-induced oxidative burst was necessary for subsequent killing of Plasmodium. Parasites incubated in microchambers separated from macrophages by a 0.45-micron filter were susceptible to H2O2 released by LK-activated macrophages incubated with PMA, opsonized zymosan, or P. yoelii antigen. Inhibition of protein synthesis by parasites exposed to products of activated macrophages was abrogated by preincubating macrophages with catalase but not with SOD, mannitol, or histidine. These results suggest that phagocytosis-associated oxidative mechanisms mediate the destruction of the malaria parasite. Hence, cell-mediated as well as antibody-dependent mechanisms cooperate in the immune response against malaria.
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PMID:Oxidative killing of the intraerythrocytic malaria parasite Plasmodium yoelii by activated macrophages. 669 Jun 6

A 7 year old boy developed in the newborn period a chronic suppurative process after routine BCG vaccination beginning at the site of the injection and spreading to the adjacent areas on neck and chin. A supraclavicular lymphadenopathy was also noted. Serial histological examinations revealed the typical histopathological pattern of tuberculosis and the boy received a tuberculostatic therapy for five years. During this time he suffered from multiple chronic bacterial infections which led to chronic granulomatous inflammations in different organs and to a fibrous pneumonitis with subsequent cor pulmonale. At the age of 6 years a negative NBT-test allowed the diagnosis of GCD. Consequently therapy with Sulfamethoxazol-Trimethoprim was started and the rate of infections diminished markedly.
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PMID:[BCG-infection in chronic granulomatous disease (author's transl)]. 718 85

Six cases of chronic granulomatous disese (CGD), three of which correspond to the X-linked genetic form and the three other to the autosomic recessive type are reported. The fact of half of the patients being females is relevant as only 24 are cited by Klebanoff and Clark in their revision in 1978. X-linked CGD: The three patients, two of them brothers, presented their first manifestations in the first year of life; in one of the BCG given at one week of life resulted in adenitis of protracted course with calcificaton. The clinical course has been very severe in two of them. At the present time the patients are 15, 11 and 9 years old. Functional studies have shown very low values in NBT tests, O2 consumption, iodination and bactericidal activity in all three. Intermediate values in the mothers and normal values in the fathers were found. Autosomal CGD: Of our three patients, two were sisters. The first manifestations appeared during the first thrimester of life. The eldest had hepatic and pulmonary granulomata at three years old. At five years, she presented an intestinal obstruction syndrome with gastric antral, duodenal and ileal stenosis caused by intramural granulomata and inflammation; she died of pneumonia shortly after. Her sister had dermatitis, hepatic abscess, pneumonia, adenitis and osteomyuelitis of the ribs; she died at six years old after a bronchopneumonia. Last patient had a sister who died at two years old affected probably gy CGD. At present our patient is 17 months old and so far had recurrent otitis, adenitis, a pneumonia and, recently, hepatic granulomata have been found. Fonctional studies in the two sisters showed similar alterations as those of the three boys. In this patient an alteration of chemotaxis of cellular origen was found as well.
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PMID:[Chronic granulomatous disease: clinical and functional studies in six cases (author's transl)]. 740 65

Mantoux test with 2 TU and tuberculin test results gave evidence for the pattern of body responses. A form of tuberculin therapy is suggested. The latter is shown to stimulate a spontaneous and BCG-enhanced NBT test (p < 0.05) and specific desensitization in the absence of humoral immunity changes (IgM, IgG, IgA and titers of circulating antituberculous antibodies).
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PMID:[Immunological bases of tuberculin therapy in combined treatment of patients with pulmonary tuberculosis]. 787 Jul 17

108 guinea pigs were infected with M-tuberculosis 2 weeks later 36 of them were put on treatment with rifampicin and isoniazid, the rest served as untreated control. The comparison was made of mixed population of all the cells isolated from bronchoalveolar lavage versus pure fraction of alveolar macrophages (AM) by spontaneous and BCG killed culture-stimulated NBT-test, activity of superoxide dismutase and catalase, levels of malonic dialdehyde. Estimations were conducted 1 day, 1, 2 and 6 weeks after inoculation in untreated animals and after 1 months of treatment in treated animals. AM lost ability for stimulation to the end of 24 h period since inoculation. 1-2 weeks later metabolic depression and complete areactivity occurred. Mixed population within postinoculation week 1 mobilized its defense potential. In extensive generalized tuberculosis all the cells of the respiratory tract worked for self-defense and lost protecting abilities. Specific chemotherapy reestablished functional status of both AM and cell population on the whole.
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PMID:[Oxidative metabolism changes in respiratory tract cells of guinea pigs during natural development of experimental tuberculosis and under specific chemotherapy]. 865 93

The aim of this work was to estimate influence of activated immune response on hematopoiesis in vitro, using the experimental model of BCG immunized BALB/c mice and in patients with chronic immunoactivation: long-lasting infections, autoimmunity or malignancy. We correlated changes in long term bone marrow cultures (Dexter) and NBT reduction with appearance of anemia in patients and experimental model of immunization by BCG. Increased spontaneous NBT reduction pointed out role of macrophage activation in bone marrow stroma damage. Long-term bone marrow cultures showed reduced number of hematopoietic cells, with predomination of fibroblasts and loss of fat cells. This results correlated with anemia and leucocytosis with stimulated myelopoiesis in peripheral blood. Activation of immune response, or acting of any agent that directly changes extracellular matrix and cellularity of bone marrow, may result in microenviroment bone marrow damage that modify hematopoiesis.
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PMID:[Bone marrow stromal damage mediated by immune response activity]. 1817 80


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