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Query: KEGG:D02003 (NBT)
 1,323 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The experiment was carried out on 50 slaughter cocks, aged 5 weeks. The purpose of the experiment was to estimate the effect of beta-carotene and apo-beta-carotenoic acid (10% Carophyll yellow) on some indices of cellular immunity in chicks receiving the feed of high vitamin A content. Chl and NBT proved that carotenoids increased phagocytic activity of neutrophiles in peripheral blood. Similarly, in the case of beta-carotene it was found by blastic transformation test that on 30-40th day of the experiment, the value IS of peripheral blood lymphocytes was increased. On the other hand, chicks fed apo-beta-carotenoic acid showed such an effect on 40th day of observation only in the case when the lowest dose of preparation (24 mg/kg of feed) and Con A as mitogen were applied. In each experimental group, the chicks exhibited increased flavin content in liver and markedly increased retinol content due to apo-beta-carotenoic acid (24 mg/kg of feed). On the other hand, the contents of alpha tocopherol and ascorbic acid did not show significant differences between experimental and control groups. Similar were the results with iron, zinc and magnesium. Copper content of the liver was markedly increased in the birds receiving apo-beta-carotenoic acid in the dose of 24 mg/kg of feed. The increased content of manganese was observed in the liver of birds receiving beta carotene (6 mg/kg of feed).
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PMID:[Effect of carotenoids on selected indicators of cellular immunity in cockerels receiving food with high level of vitamin A]. 213 44

A novel assay was developed to measure the capacity of polyphenols to chelate cobalt(II) by using the reduction of the tetrazolium salts, NBT (nitroblue tetrazolium chloride), MTT (methylthiazolyldiphenyl-tetrazolium bromide), and XTT (2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide) to formazan products. The reduction of the salts is initiated by a cocktail comprised of cobalt(II), H(2)O(2), and selenium(IV), which generates hydroxyl radical, peroxide, and superoxide ions. However, because cobalt(II) could not be replaced either by Fe(II), Mn(II), or Cu(II), the classical Fenton transitional metals, it indicates that cobalt is the key player in the tetrazolium salt reduction. Micromolar concentrations of a large variety of antioxidant polyphenols and minute amounts of fruit beverages rich in polyphenols can readily chelate cobalt, resulting in the inhibition of the reduction of tetrazolium salt to formazan, in a dose-dependent manner. However, this method is unsuitable to measure low molecular weight antioxidants such as ascorbate, uric acid, and vitamin E since these have no chelating properties for cobalt(II). The newly described tetrazolium reduction method is as sensitive as the ferric ion reducing antioxidant power, 2,2-diphenyl-2-picrylhydrazyl hydrate, and the luminol-dependent chemiluminescence antioxidant assays. The practical advantages of using the newly described method to quantify polyphenol levels from various sources are briefly discussed.
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PMID:A cobalt-based tetrazolium salts reduction test to assay polyphenols. 1972 2

Five kinds of Salen Mn(II) and two kinds of Eu(III) complexes were synthesized. Under the system of NBT/L-methionine, which creates and detects O2*-, the complexes of antioxidant activity were studied, and the conclusion revealed that all Mn(II) complexes have obvious antioxidant activity. The fluorescence study indicated that all the Salen metal complexes could bind with DNA, which shows their potential anticancer activity.
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PMID:[Antioxidant activity and spectroscopic behavior study with DNA of several Salen Mn(II), Eu(III) complexes]. 2067 39

Complexes of manganese(II), cobalt(II), nickel(II), copper(II) and zinc(II) with a Schiff base, formed by the condensation of isatin with 2-aminopyrimidine have been synthesised and characterised through elemental analysis, molar conductance measurements, magnetic susceptibility, IR, UV-Vis, (1)HNMR, FAB mass and EPR spectral studies. The spectral data revealed that the ligand acts as neutral bidentate, coordinating to the metal ion through the carbonyl oxygen and azomethine nitrogen. Molar conductance values adequately support the electrolytic nature of the complexes. On the basis of the above observations the complexes have been formulated as [M(ISAP)2]X2, where M=Mn(II), Co(II), Ni(II), Cu(II) and Zn(II); X=Cl, OAc; ISAP=2-[N-indole-2-one]aminopyrimidine. The ligand and copper(II) complex were subjected to X-ray diffraction studies. The DNA cleavage study was monitored by gel electrophoresis method. The superoxide dismutase (SOD) mimetic activities of the ligand and the metal complexes were checked using NBT assay. The in vitro antibacterial activity of the synthesized compounds has been tested against gram negative and gram positive bacteria.
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PMID:Synthesis, spectroscopic characterisation, DNA cleavage, superoxidase dismutase activity and antibacterial properties of some transition metal complexes of a novel bidentate Schiff base derived from isatin and 2-aminopyrimidine. 2405 Dec 84