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Query: KEGG:D02003 (
NBT
)
1,323
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Using the rat bladder carcinoma cell line
NBT
-II we showed that collagens but not laminin and fibronectin were able to induce cell scattering.
Acidic fibroblast growth factor
and transforming growth factor alpha also promoted
NBT
-II cell dispersion on glass or tissue culture plastic. We have now further analysed the scatter response to these two growth factors in the presence of extracellular matrix molecules. In the presence of growth factors, no peripheral single-cell dispersion occurred on fibronectin and laminin, although time-lapse video analyses revealed intense cell mingling and motility inside the monolayer forming around
NBT
-II aggregates. Patterns of strings or files of cells protruding from the monolayer were often observed. The presence of a scattering activity in the complex acellular extracellular matrix deposited by
NBT
-II cells themselves strongly suggested that substratum conditioning was responsible for this effect. On the other hand, the two growth factors accelerated collagen-mediated
NBT
-II individual cell dispersion and locomotion in a reversible way. As a marker of cell dissociation, we studied desmosome distribution in aggregate cultures: desmosomes were present in aggregates formed in suspension even in the presence of growth factors, whereas internalization occurred after cell-to-substratum contact. On laminin or fibronectin and in the presence of growth factors, peripheral cells inside the halo of
NBT
-II aggregates did not exhibit desmosome linkages. These observations suggest that scatter effects per se are dependent on the composition of the extracellular matrix. In particular, on a substratum nonpermissive for direct cell translocation, individual cell dispersion can be replaced by en bloc patterns of migration following substratum conditioning by the cells.
...
PMID:Combined effects of extracellular matrix and growth factors on NBT-II rat bladder carcinoma cell dispersion. 172 17
Acidic fibroblast growth factor
(
aFGF
) or transforming growth factor-alpha (TGF-alpha), in addition to being mitogenic, induce individual scattering of
NBT
-II rat bladder carcinoma cell clusters on tissue culture dishes, suggesting that they may contribute to tumor cell dissemination. To assay their scattering potential and their effect on cell invasiveness in a more complex and physiologically relevant model, we analyzed the behavior of
NBT
-II spheroids confronted with urinary bladder in organotypic cultures.
NBT
-II spheroids progressively replaced the urothelium at the site of contact with the bladder explant. In the absence of
aFGF
or TGF-alpha, inserted cells grew in a pattern suggestive of local hyperplasia, with occasional invasive cell protrusions. Exogenous scattering growth factors elicited a more rapid appearance of these protrusions, which were also more numerous.
NBT
-II cells transfected with cDNA constructs bearing the gene of
aFGF
, TGF-alpha or the oncogene hst/KFGF were also used. After exogenous or autocrine stimulation of
NBT
-II cells with the growth factors, a deeper penetration of the bladder wall in the form of nodular outgrowths and clusters of infiltrating cells was always observed. Altogether these observations suggest that the stimulation of
NBT
-II clusters by scattering/growth factors can promote cell shedding and amplify invasiveness in the complex extracellular environment of bladder tissues.
...
PMID:Amplification of invasiveness in organotypic cultures after NBT-II rat bladder carcinoma stimulation with in vitro scattering factors. 172 9
