Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: KEGG:D02003 (NBT)
 1,323 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Techniques for the ultrastructural demonstration of dehydrogenases in cerebral cortex are described. The best fixation for good fine structural preservation and retention of LDH and NADH-diphorase was obtained by perfusion with a misture of formaldehyde and glutaraldehyde and for SDH by perfusion with formaldehyde. Comparison of incubation conditions showed that consistent results were obtained using enzyme markers NBT and DS-NBT for LDH and NADH-diaphorase: DS-NBT was more satisfactory than NBT and BSPT for SDH. Penetration of incubation media was improved by Triton X-100: DMSO and ultrasonic treatment were less effective. The techniques enabled the first electron cytochemical demonstration of dehydrogenases in different elements of prefixed cerebral cortex. Ultrastructural sites of enzyme activities were localized within cristae and inter-membrane spaces of mitochondria in nerve cell cytoplasm and its processes, oligodendrocytes and astrocytes. Authenticity of the ultrastructural sites was confirmed by four different control experiments.
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PMID:Ultrastructural demonstration of dehydrogenases in rat cerebral cortex. 47 91

In 73 workers producing rubber mixtures and tubes the number and percentage composition of white peripheral blood cells as well as the NBT reduction test and cytochemical reactions to lactic (LDH) and succinic (SDH) dehydrogenases in neutrophils were estimated. The amount and percentage composition only slightly differed from controls. The NBT reduction index and the reactivity of LDH and SDH tests in neutrophils were elevated.
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PMID:[Peripheral blood neutrophils in automobile tire factory workers in the light of cytochemical studies]. 340 85

In the stem phloem of Cucurbita pepo the enzymes GAPDH, ADH, MDH, NADP-IDH, NAD-IDH, G6PDH and SDH were localized histochemically with the aid of tetrazolium salt (NBT). When the stems were deep-frozen the most intense formation of formazan was found in companion cells, less in phloem parenchyma cells, and very little in sieve tubes.The distribution of enzymes in phloem markedly changes when stems were cut 2 minutes before freezing: 2,5 cm behind the sectional area little formazan was found. Companion cells and parenchyma cells had lost nearly all activity. 15 cm behind the sectional area there was again a higher concentration of formazan in the companion cells and parenchyma cells. In this region an even higher activity was detected in sieve tubes. 25-30 cm behind the sectional area the distribution of formazan was nearly the same as in the intact stems.Apparently activities of the enzymes tested primaily occur in the companion cells and parenchyma cells only. After wounding they are translocated into sieve tubes or exudate.
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PMID:[Localization of dehydrogenases of energy metabolism in the pholoem of Cucurbita pepo L]. 2446 72