Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: KEGG:D02003 (NBT)
 1,323 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The chymotryptic activity was assessed in 40 newborns and infants age 3-180 days using the NBT-PABA test. Nine newborns were studied serially at different age periods. A group of 18 cystic fibrosis patients and a group of 17 healthy children served as pancreatic insufficient and pancreatic sufficient controls, respectively. The results demonstrate a gradual increase with age of chymotryptic activity, approaching the levels of older children at about 180 days. The newborns who were studied serially demonstrated an individual pattern of increase in their chymotryptic activity. In the first days of life, newborns show low chymotryptic activity similar to that found in cystic fibrosis patients.
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PMID:The development of the chymotryptic activity during postnatal life using the bentiromide test. 325 44

The oral D-xylose- and NBT-PABA-test are frequently applied as screening methods for malassimilation syndromes. After oral load time-related serum concentrations are used as reference values. In this study both substances were given simultaneously as isotonic solutions after a fasting period of 6 hours. A standard meal was offered 20 minutes later. Blood samples were taken prior to and 60, 90 and 120 minutes after administration of the test-substances. 43 pediatric patients were studied. 11 patients with cystic fibrosis, 10 patients with underlying intestinal disease such as coeliac disease and cows milk intolerance and 22 patients with other than gastrointestinal diseases were compared. In children with coeliac disease and cows milk intolerance 1-hour PABA concentrations were in the range of the control group whereas 1-hour D-xylose concentrations were lower than 15 mg/dl. In patients with cystic fibrosis the maximum concentrations of D-xylose were within the limits of normal values while 1-hour PABA concentrations were lower than 0.15 mg/dl in cases of relevant exocrine pancreatic insufficiency. The results of this study show that the combined D-xylose and NBT-PABA-test has proved its practical value in cases of suspected malassimilation and its discriminatory property between patients with relevant pancreatic insufficiency and patients with diminished intestinal absorptive capacity due to coeliac disease or cow's milk intolerance.
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PMID:[Kinetics and clinical use of the combined D-xylose and NBT-PABA test]. 350 Mar 62

We describe and evaluate two frequently used indirect methods for assessing exocrine pancreatic function: the N-benzoyl-L-tyrosyl-p-aminobenzoic acid test (NBT-PABA) and the pancreolauryl test. In both procedures, the patient is orally administered a substrate that is metabolized into two or more products by pancreatic enzymes. At least one of the reaction products is absorbed from the gut, conjugated, and excreted in urine, where it can be measured. Both tests can be used in the diagnosis and monitoring of cystic fibrosis, chronic pancreatitis, and pancreatic carcinoma, and in monitoring pancreatic enzyme replacement therapy to determine the appropriate dose. In comparison with the NBT-PABA procedure, the pancreolauryl test seems to have better specificity and sensitivity, undergoes almost no interference from other drugs or serum compounds, requires no complex hydrolytic conditions, and is independent of renal function.
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PMID:Two indirect tests of exocrine pancreatic function evaluated. 354 96

The neutrophil function of 12 patients with cystic fibrosis was evaluated via the new Immunobead/NBT dye reduction test. While the neutrophils appeared to be hyperfunctional when studied in the standard assay using fetal calf serum, we demonstrated a significant neutrophil dysfunction when studied in their own respective serum. The dysfunction involved both the phagocytic and intracellular killing ability of the cells, which we postulate is due to an inhibitory factor present in the patient's serum. The inhibitory factor did not appear to be related to the level of circulating immune complexes.
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PMID:Demonstration of neutrophil dysfunction in the serum of patients with cystic fibrosis. 728 70

We have used a double-stranded DNA probe linked to the cystic fibrosis locus to detect a single-copy gene from varying amounts of genomic DNA, with Southern blot analysis. The DNA plasmid probe was labeled with either biotin or digoxigenin. Biotin or digoxigenin was then linked to alkaline phosphatase (ALP) with use of streptavidin or anti-digoxigenin antibodies, respectively. ALP activity was then detected with a chromogenic (BCIP/NBT), chemiluminogenic (AMPPD), or fluorogenic (HNPP) substrate. Our results suggest that biotin and digoxigenin perform similarly and that the three substrates exhibit similar detectability under appropriate substrate incubation times: 20-120 min (AMPPD), 2-12 h (HNPP), and 18-48 h (BCIP/NBT). Under optimised conditions and the probe used, these methods detect single-copy genes from as little as 0.3 micrograms of total genomic DNA.
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PMID:Evaluation of nonisotopic labeling and detection techniques for nucleic acid hybridization. 850 46