Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: HUMANGGP:021525 (albumin)
 60,984 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Tiny (0.2% TBS), partial thickness, non-contact radiant heat burns in guinea pigs resulted, within 3 hours, in significant edema formation and protein leakage at the site of the injury. Areas of skin distant to the burn also showed an increase in water content but no protein leakage. Pretreatment of the animals with either chlorisondamine hydrochloride or a mixture of methysergide and chlorpheniramine significantly decreased postburn edema formation and protein leakage. Liquid emulsion autoradiography revealed that leakage of protein occurs primarily in the areas of skin adjacent to the panniculus carnosus. The studies suggest that: the increase in vascular permeability that occurs as a consequence of burn injuries is humorally mediated; albumin leakage is limited to the injured tissues; and histamine, serotonin, and presumably catecholamines play significant roles in the development of this phenomenon.
 ...
PMID:Effect of antihistamine-antiserotonin and ganglionic blocking agents upon increased capillary permeability following burn trauma. 0 May 15

A discussion of causes, diagnosis, and treatment of childhood diarrhea is presented. If the pH of the stomach is not acidic enough, harmful bacteria may not be killed. If peristaltic action is not regular, defense against intestinal diseases is hindered. The proper bacteria and immunologlobulin (with secretory piece and J-chain) must be present in the intestine to prevent infection. Loss of large volumes of fluid is one clinical symptom of diarrhea. The changes in water volume can cause changes in the osmolarity of other body fluids, which can lead to fluid loss in other organs or increase in brain pressure. Acidosis can also accompany diarrhea, as can intracellular losses of potassium and other ions. Laboratory tests of the blood for hemoglobulin, hematocrit, and electrolyte levels can aid in the diagnosis of the cause of infantile diarrhea. The feces can be tested for leukocytes, erythrocytes, and pH value. Absence of leukocytes can indicate an enterotoxic or viral diarrhea; antibiotics are contraindicated. Determinations of E. coli and enterotoxins as well as immunoglobulin tests do not provide relevant information. Treatment can be effected by oral glucose-electrolyte therapy. In more severe cases, i.v. infusions of salt, plasma, salt-free albumin, or whole blood may be indicated. Medications such as ampicillin and clindamycin have been used to treat diarrhea, but any medication which inhibits peristaltic action is contraindicated in children.
 ...
PMID:[Past and present aspects of diarrheal disease in childhood. Clinical study and treatment (author's transl)]. 0 46

We conducted a controlled, prospective trial to evaluate the effectiveness of rapidly infusing sodium bicarbonate (NaHCO3) and salt-poor albumin into high-risk, premature infants in the first 2 hours of life. Fifty-three infants, randomized into one of four treatment groups, received 8 ml. per kilogram of a solution containing either (A) glucose in water, (B) salt-poor albumin, (C) NaHCO3, or (D) a combination of albumin and NaHCO3. After the initial infusion, the babies received no colloid or alkali solutions until 4 hours of age. We managed them supportively with warmth, appropriate oxygen administration, isotonic fluid infusion, and close monitoring. Among the infants who received alkali, 14 of 26 acquired the respiratory distress syndrome (RDS), 11 died, and four had intracranial hemorrhage. Among babies who received no alkali, RDS occurred in 11 of 27, 5 died, and none had intracranial hemorrhage. These results do not support the common practice of rapidly infusing NaHCO3 into high-risk, premature infants, and they suggest that the early management of such infants needs renewed critical evaluation.
 ...
PMID:Rapid infusion of sodium bicarbonate and albumin into high-risk premature infants soon after birth: a controlled, prospective trial. 2455 88

11-Fold purified protease preparation is isolated from cultural medium of Torula thermophila UzPT-1 by means of ammonium sulphate precipitation and gel chromatography through Sephadex G-100. Disc polyacrylamide gel electrophoresis revealed two portease components, one of them possessing proteolytic activity. pH interval for protease activity was found to be 3.5-12, the maximal activity was observed at pH 8.5-11, the highest enzyme resistance--at pH 6-8. The enzyme almost completely preserved its activity for 1 hour in distilled water at 60 degrees C. The temperature maximum of the enzyme activity was 70 degrees at pH 8. The enzyme may be referred to proteases of serine nature, because it is completely inactivated with diisopropylphosphofluoridate, but it retains the activity in the presence of chelating agents (EDTA, o-phenantroline, ditizone) and inhibitors of SH-groups (sodium p-chloromercuriumbenzoate, iodoacetic acid). The enzyme was not inactivated with phenylmethylsulphonylfluoride and the trypsin inhibitor from soybean. The protease studied most efficiently hydrolyzed caseine and hemoglobin, in a less degree--human serum albumin and fibrinogen and almost did not attack egg albumin. The enzyme undergoes association-dissociation under pH change during gel filtration through Sephadex.
 ...
PMID:[Partial purification and properties of protease from Torula thermophila]. 0 27

The hydrolysis of the phospholipids of intact human serum high density lipoprotein 3 (HDL3) by pure alpha-phospholipase A2 from Crotalus adamanteus was studied by pH-stat titration. The enzyme quantitatively hydrolyzed phosphatidylcholine and phosphatidylethanolamine and left sphinogomyelin intact, yielding a stable and water-soluble modified HDL. Lysophospholipids and free fatty acids, the products of hydrolysis, remained in the lipoprotein. When 1 mol of defatted bovine serum albumin/mol of substrate phospholipids was added to the reaction mixture, up to 60% of the fatty acids and 85% of the lysophospholipids were removed from the modified lipoprotein. The immunological reactivity of the hydrolyzed HDL remained unaltered in both the presence and absence of albumin. The changes in the physical properties of the lipoprotein during hydrolysis were rather small, the most notable being an increase in the hydrated density and in the electrophoretic mobility in alkaline buffers. The hydrolysis followed an apparent first order time course with product inhibition (KI) and yielded values of kcat/Km = 7 X 10(5 M(-1)s(-1) and KI congruent to 1 X 10(-4) M. Addition of albumin to the reaction mixture relieved the product inhibition without any alteration of the kinetic parameters. High concentrations of albumin protected some of the substrate phospholipids from hydrolysis, presumably through complexation to the lipoprotein. The Arrhenius plot for the experimental first order rate constant in the absence of albumin (kexp = kcat (KI/Km)) was linear between 15 degrees and 47 degrees, indicating the absence of any phospholipid phase transitions and yielding an activation energy of 15.2 kcal/mol. From the accessibility of the HDL phospholipids to phospholipase A2 one concludes that the phosphatidylcholine and phosphatidylethanolamine are located at, or are in rapid equilibrium with, the surface of this lipoprotein. It also appears that these phospholipids are not essential for maintaining the supramolecular properties of the lipoprotein in vitro. Thsu the study of the modified Hdl should provide valuable information concenring the structure and function of this lipoprotein particularly with regard to the role played by shiingomyelin.
 ...
PMID:Kinetic study of the action of snake venom phospholipase A2 on human serum high density lipoprotein 3. 0 36

A method for covalent coupling of bilirubin to albumin is described. Human serum albumin-bilirubin (1:1 complex) has been treated with water soluble carbodiimide in order to obtain covalent coupling of bilirubin to albumin. The reaction conditions have been varied with respect to pH, reaction time and concentration of reagent to obtain the optimal coupling. The prepared albumin-bilirubin compounds were investigated by spectrophotometry, gel filtration and gel electrophoresis to ascertain the covalent nature of the bond and to characterize the products further. Gel electrophoresis and gel filtration showed that a monomer fraction could be prepared, and this fraction was a suitable material for further studies.
 ...
PMID:Covalent coupling of bilirubin to albumin. 0 97

All hypervolemia or any decrease in plasma oncotic pressure leads to deterioration in lesional pulmonary edema. The aim of albumin infusions is to restore the plasma oncotic pressure and to oppose the passage of water into the extravascular space. This therapy is however debatable owing to the abnormal increase in the permeability of the alveolo-capillary membrane to proteins. Diuretics enable one to lower the volemia and to maintain the pulmonary capillary pressure at its minimum level. However, strict hemodynamic supervision is absolutely necessary. Cortico-steroids have been recommended without serious physiopathological justification. However, they have a preventive action on interstitial edema in endotoxic shock.
 ...
PMID:[Reduction and anti-inflammatory technics in non-hemodynamic pulmonary edemas]. 0 65

Studies were performed to evaluate the effects of the chronic administration of furosemide on hydrogen and electrolyte excretion in dogs on a normal electrolyte diet and in the absence of electrolyte or volume depletion. Control daily excretion in five dogs averaged 64 meq for Na, 51 meq for K, 66 meq for Cl, and 17 meq for net H. Furosemide, 40 mg, in the drinking water 3 times daily was given for 4 days. On day 1 Na excretion averaged 128 meq, but thereafter was not significantly different from control levels. Over 4 days cumulative net H excretion increased 63.6 meq and plasma HCO3 rose 6.6 meq/liter. The same dogs were restudied by the same protocol except that, to obviate electrolyte depletion, NaCl and KCl were administered daily in quantities sufficient to replace urinary losses. All dogs remained in positive Na, K, and Cl balance. Body weight, hematocrit, plasma albumin, creatinine, and plasma renin activity were unchanged, indicating the absence of electrolyte or volume depletion. Nonetheless, cumulative net H excretion increased 61.2 meq and plasma HCO3 increased 4.3 meq/liter. Two adrenalectomized dogs receiving steroid replacement showed similar changes in net H excretion and plasma HCO3. These experiments suggest that chronic furosemide administration may enhance H excretion and generate alkalosis even in the absence of volume or electrolyte depletion and without increased aldosterone secretion.
 ...
PMID:Effect of chronic furosemide administration on hydrogen and sodium excretion in the dog. 1 99

Ethanol decreases hepatic protein and albumin synthesis, and inhibits pancreatic water, bicarbonate, and protein secretion. Since these actions of ethanol are opposite to those reported for secretin, cholecystokinin-pancreozymin, and pentagastrin which may be mediated through increases in cyclic GMP, it appeared possible that the inhibitory actions of ethanol might be mediated through inhibition of guanylate cyclase, the enzyme that catalyzes the production of cyclic GMP. Ethanol inhibited soluble preparations of guanylate cyclase from rat liver, pancreas, stomach, and ileum. Maximal inhibition was observed at 5.0 and 2.5 percent ethanol. The inhibitory effects of ethanol on the guanylate cyclase-cyclic GMP system of these tissues provide a possible explanation for some of the diverse effects of ethanol on these tissues.
 ...
PMID:Ethanol-induced inhibition of guanylate cyclase in liver, pancreas, stomach and intestine. 1 94

The protease isolated jawasee shrub was found to hydrolyze egg albumin, casein, haemoglobin and gelatin optimally near neutral pH. Fibrin, bovin serum albumin, skin albumin and skin mucoids were hydrolyzed at slightly alkaline pH, while skin globulins were hydrolyzed at slightly acidic pH. The enzyme had no effect of fibrous collagen. The optimum conditions for the hydrolysis of 50 mg of egg albumin were found to be 50 mg of alhagain at pH 6.0 and 45 degrees C for 30 minutes. A Km value of 4.4 X 10(-3) M was obtained from the Lineweaver-Burk plot for the hydrolysis of egg albumin. The enzyme was found to be comparatively thermostable and was most stable at pH 4.7. Ultraviolet irradiation exhibited no appreciable effect on the enzyme activity. The ultraviolet absorption spectrum of alhagain in bi-distilled water resembles those of bromelain and trypsin. The sugar-containing enzyme was found to have a molecular weight of 20,650. The enzymeconsists of 189 amino acid residues per molecule, neutral and acidic amino acids being present in high concentrations. The partial specific volume of alhagain was calculated to be 0.743 ml/g from its amino acid composition. Phenylalnine and arginine formed the amino terminal amino acids of alhagain, while aspartic acid and serine were identified as its carboxy terminal amino acids. Results are discussed with relation to other plant proteases.
 ...
PMID:Studies on the physico-chemical properties of alhagain. 2 Nov 47


1 2 3 4 5 6 7 8 9 10 Next >>