Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: HUMANGGP:008114 (TEM)
 20,717 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

beta-Lactamase activity was detected in cell-free preparations obtained from ultrasonic treatment of Neisseria gonorrhoeae after growth in liquid medium. Crude preparations of beta-lactamase were subjected to affinity chromatography, using several beta-lactam antibiotics as ligands bound to agarose supports. Affinity gels produced by coupling 7-aminocephalosporanic acid or 6-aminopenicillanic acid by their amino groups to carboxyl-terminal agarose via a five- to eight-carbon spacer arm proved to be effective chromatography media. beta-Lactamase preparations subjected to chromatography using these gels were purified 200-fold, with approximately 80% recovery of active material. Purified preparations were judged homogeneous by their behavior during electrophoresis on polyacrylamide in both the presence and absence of sodium dodecyl sulfate. Characterization of the purified enzyme established a molecular weight of approximately 25,000 and an isoelectric point of 5.4. Analyses of substrate specificity, effect of inhibitors, pH, and kinetic parameters were performed. The evidence suggests that the beta-lactamase produced in N. gonorrhoeae closely resembles the character of class IIIa (TEM-type) beta-lactamases.
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PMID:Purification by affinity chromatography and properties of a beta-lactamase isolated from Neisseria gonorrhoeae. 10 75

A method for unmounting entire resin-embedded samples for SEM observation is described. This technique is particularly useful when correlation of TEM and SEM images is desired for material that is no longer available for conventional SEM preparative procedures. Sample embedded in a variety of epoxy-type resins were trimmed of excess resin and placed in a concentrated solution of sodium methoxide. After complete dissolution of the resin, the tissue was washed in a graded series of sodium methoxide in methanol--benzene, transferred to acetone, critical point dried, mounted on stubs, and coated with gold-palladium. Upon viewing in the SEM, the tissue sample showed remarkable preservation of detail at relatively high magnifications.
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PMID:A method for the removal of epoxy resins from tissue in preparation for scanning electron microscopy. 35 29

Bidirectional fluxes of Na+, K+ and Ca++ were estimated under in vitro conditions through isolated patches of rat peritoneal membranes (parietal and visceral). Morphological (TEM) and functional (changes in fluxes) data are presented to show that mesothelium lining the parietal peritoneum forms a barrier than limits diffusion of ions across this membrane. In addition, it was found that Furosemide and nitroprusside change the permeability of the mesothelium for sodium or potassium, respectively.
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PMID:Evaluation of the participation of parietal peritoneum in dialysis: physiological, morphological and pharmacological data. 54 77

Patients with chorea-acanthocytosis exhibit symptoms of self-biting, choreic movement, and acanthocytosis, but not dementia. The mechanism of choreic movements is still unknown. In order to clarify the etiologic mechanism underlying these movements, we evaluated the erythrocyte membrane in one patient with chorea-acanthocytosis. A 35-year-old female was admitted to Saitama Medical School Hospital because of involuntary movements. She was alert, well-oriented, and had no gross memory defects. She had slurred speech, choreic movements and lip biting. Laboratory examination showed acanthocytes in her peripheral red blood cells, normal serum lipid values, and caudate atrophy on her brain CT scan. In analyzing the acanthocytes, we initially evaluated the size of the acanthocyte population by incubating her red blood cells with plasma. The cell population approximately doubled after 2 hours incubation. Next we examined the protein composition of erythrocyte ghost by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). There was no significant difference between the patient's erythrocyte ghosts and those of a control. Then we investigated morphological changes in the patient's erythrocyte by scanning and transmission electron microscopy (SEM and TEM). SEM showed the typical acanthocyte shape. The quick-freeze, freeze-substitution method confirmed that the routine TEM section was not artifactual, and was in fact in accurate reflection of the actual features of acanthocytes. TEM of the sections prepared from erythrocyte ghosts demonstrated that spectrin tended to be accumulated in the thorn region. Furthermore, TEM of quick-freeze, deep-etched replica of the ghost revealed more clearly a spectrin network densely packed on the inner hydrophilic surface.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:[Studies on the erythrocyte membrane skeleton in a patient with chorea-acanthocytosis--theoretical speculation on the mechanism of neurological involvement]. 141 52

Point mutation in the nucleotide sequence of the structural genes for the TEM-type penicillinases can broaden their substrate spectrum towards all beta-lactams except cephamicins and imipenem. We describe here hybridization techniques for the detection of point mutations by non-radioactive oligonucleotide probes with plasmid DNA carrying bla T genes immobilized in polystyrene microwells. After hybridization in discriminating conditions with corresponding biotinylated oligonucleotide probes, the hybrids were detected by using a streptavidin-alkaline phosphatase conjugate and a fluorogenic substrate, 4-methylumbelliferyl-phosphate. The adsorption of DNA to microwells used in the present work was found to be independent of Mg2+ and Na+ concentrations. By this method, less than 3 fmols of target DNA were sufficient for the detection of point mutation.
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PMID:Detection of point mutation in bla T genes of Enterobacteriaceae by biotinylated oligonucleotide probes using microwell hybridization and enzymofluorometric method. 154 33

In this study the morphologic and functional changes were compared after irradiation (single dose, 15 Gy) of rat submandibular salivary glands. Before and 1-10 days after local irradiation of the gland region, samples of submandibular saliva were collected after stimulation by pilocarpine. At the same time-points and also 3 h postirradiation submandibular glands were carefully extirpated and prepared for histocytologic examination (LM, TEM). Maximal increase of the lag phase and decrease of the flow rate were observed 3 days after irradiation, while [K+] and [Na+] increased and decreased, respectively, from days 1 and 3 after irradiation. Morphologic changes were observed from the third hour after irradiation, were maximal 3 days after irradiation and had partially recovered by day 10. Three hours and 1 day after irradiation degranulation of convoluted granulated tubes (CGT) was observed. Three days after irradiation the most striking morphologic changes in serous and mucous cells were distension of the cisternae of the RER, degeneration of mitochondria and vacuolization of the cytoplasm. Fibril-like condensations of electron dense material in the mucous granules were observed 3 h, 1 and 6 days after irradiation. Regranulation of CGT cells was observed from day 6. From this study it is concluded that changes in salivary gland function can be observed before major morphologic changes occur. Functional changes persist after the morphologic changes seem to have virtually returned to normal.
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PMID:Acute irradiation effects on morphology and function of rat submandibular glands. 180 90

This study examines prostacyclin production by blood-contacting surfaces within woven vascular prostheses of polydioxanone (PDS), polyglactin 910 (PG910), or Dacron interposed into rabbit infrarenal aortas. Grafts and normal aortic segments were explanted after 1, 3, and 6 months for pulsatile perfusion with Medium-199 for 60 minutes. Aliquots were removed serially for 6-keto-PGF1 alpha assay. After 30 minutes sodium arachidonate (10 micrograms/ml) was added. Specimens were studied by light microscopy, SEM and TEM. Patency in all three groups exceeded 90%. All three showed re-endothelialization at one month. Normal aorta produced low basal 6-keto-PGF1 alpha with a marked evanescent post arachidonate increase. Dacron did not differ from normal aorta. PG910 and PDS both produced significantly less 6-keto-PGF1 alpha post arachidonate at one month but both increased to normal by three months.
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PMID:Prostacyclin production by blood-contacting surfaces of endothelialized vascular prostheses. 214 73

The perilymphatic space was perfused with artificial perilymph containing 5 x 10(-3)M potassium canrenoate. The EP, K+ and Na+ activities in the scala media were measured with double barrelled K+ or Na+ selective microelectrode. Following the onset of the perfusion, the EP gradually declined and was stable after about 20 minutes. K+ activity also declined gradually but Na+ activity was unchanged. When the EP became stable, the artificial ventilation was stopped. The EP dropped to a large negative potential and K+ activity decreased gradually, but Na+ activity increased by degrees. The same results were observed in the untreated animals when the ventilation was stopped. There are no pathological changes both in the TEM view of the stria vascularis and in the SEM view of the hair cell. These results suggest that this drug may affect K+ conductance of the stria vascularis specifically.
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PMID:[Effect of potassium canrenoate on the EP and Na+, K+ activities in endolymph]. 235 44

The effect on the tegument of adult Fasciola hepatica of incubation in the sodium ionophore monensin, the Na+/K+-ATPase inhibitor ouabain and ouabain pretreatment followed by monensin has been determined in vitro by scanning and transmission electron microscopy (SEM, TEM). With monensin incubation alone (1 x 10(-6) M), a flattening of the tegument with some loss of spines on the ventral surface is evident from 0.5 h onwards. Internally, the subtegumental musculature becomes grossly swollen, although there is no swelling of the infoldings of the basal plasma membrane of the tegument, even after 24 h incubation. Ouabain incubation (1 x 10(-3) M) induces folding of the apical surface of the tegument from 0.5 h onwards, and this is accompanied by the formation of blebs and microvilli. Brief (0.5 h) exposure to ouabain (1 x 10(-3) M) followed by monensin treatment (1 x 10(-4) M, 3 h) leads to gross "vacuolation" of the tegument, but this is not due to swelling of the basal infoldings. The other main feature of ouabain-pretreated flukes is the projection of basal lamina-like material into the tegumental syncytium. Monensin treatment alone (1 x 10(-6) M) results in the Golgi complexes of the tegumental cells becoming very diffuse from 1.5 h onwards, and relatively few secretory bodies are present in the cytoplasm. After 0.5 h incubation in ouabain (1 x 10(-3) M), the Golgi complexes of the tegumental cells are indistinct, although numerous secretory bodies are still present. The classical monensin-induced swelling of the Golgi cisternae is observed in the tegumental cells only when monensin treatment (1 x 10(-4) M, 3 h) was preceded by brief (0.5 h) exposure to ouabain (1 x 10(-3) M). The results are discussed in relation to the postulated osmoregulatory role of the tegument and the role of sodium pumps in membrane function in the fluke.
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PMID:Fasciola hepatica: the effect of the sodium ionophore monensin on the adult tegument. 254 Apr 90

Attempts to promote crystal growth in maturation stage enamel from rat incisors were carried out by incubation in saturated solutions of calcium phosphate. Resulting crystallites were visualised in the TEM and the dimensions of their profiles measured. No crystal growth was observed unless the maturation stage enamel was first pretreated with either 8M urea or sodium hypochlorite to remove residual protein matrix. The results suggest that the protein matrix plays an important role in the control of crystal growth in vivo.
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PMID:Control of crystal growth during enamel maturation. 259 65


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