Gene/Protein Disease Symptom Drug Enzyme Compound
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 11,742 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The effects that the aminoglycoside-aminocyclitol antibiotics amikacin, dihydrostreptomycin, gentamicin, neomycin, and spectinomycin, the neomycin fragment neamine, and the polybasic compounds spermine and poly-L-lysine, have on outer hair cells in cochlear cultures prepared from the early post-natal mouse have been assessed using both scanning and transmission electron microscopy (SEM and TEM). The antibiotics were used at concentrations ranging from 0.25-1.0 mM, spermine from 10 microM to 3.0 mM, and poly-L-lysine from 0.05-2 microM. Qualitative assessment of apical surface damage allows the antibiotics to be ranked in the following order: neomycin > gentamicin > dihydrostreptomycin > amikacin > neamine > spectinomycin. At a concentration of 1 mM spectinomycin is essentially non-toxic and the effects of neamine are marginal. Poly-L-lysine and spermine also cause surface damage, with poly-L-lysine being substantially more toxic than any of the antibiotics, and spermine ranking, on the basis of SEM observations, between dihydrostreptomycin and amikacin. TEM indicates that although all toxic compounds cause damage to the apical surface of the hair cell, only neomycin, poly-L-lysine and spermine induce the formation of whorls of tightly packed membrane resembling myelin within the apical surface lesions to any great extent. Apical-surface changes induced by dihydrostreptomycin and amikacin are simply large distensions of the cell filled with cytoplasmic organelles of normal appearance. Although the effects of the aminoglycoside antibiotics are largely limited to the apical surface of the cell, poly-L-lysine induces complete necrosis of the cell, and spermine causes a dramatic increase in cytoplasmic electron density and condensation of the nuclear chromatin.
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PMID:Ototoxicity in vitro: effects of neomycin, gentamicin, dihydrostreptomycin, amikacin, spectinomycin, neamine, spermine and poly-L-lysine. 751 88

Poly(N-isopropylacrylamide) (PNIPAM) microgel particles dispersed in water have been used as a matrix for the polymerization of a hydrophobic monomer, N-methylpyrrole (MPy). The presence of poly(MPy) (PMPy) within the dried composite particles has been confirmed using electron paramagnetic resonance (EPR) measurements which show a characteristic free-radical signal at g=2.007. Electron microscopy data (TEM) show that the composite PNIPAM-xPMPy particles have a "raspberry-like" morphology. (The value for x represents the volume percent of MPy added during synthesis with respect to the total microgel volume.) PCS data indicate that PMPy occupies the majority of the collapsed composite particle volume. The maximum value of x consistent with colloid stability for PNIPAM-xPMPy dispersions is 4.5%. Higher values of x result in coagulation due to interparticle bridging by PMPy. Variable temperature PCS measurements of the PNIPAM-xPMPy dispersions have been used to study the thermally induced collapse of the composite particles. The extent of collapse becomes less with increasing values for x. The embedded PMPy particles restrict the extent of PNIPAM network contraction. The stability of the PNIPAM-4.5PMPy dispersions was investigated by means of turbidity measurements using aqueous 0.1 M NaCl solution. The upper critical flocculation temperatures (UCFT) for PNIPAM and PNIPAM-4.5PMPy dispersions were identical (38-39 degrees C). The flocculation observed was found to be fully reversible. The composite dispersion stability in the absence of salt was attributed to electrosteric stabilization afforded by the PNIPAM matrix. These results indicate that PNIPAM microgel particles may have application as a matrix for the polymerization of hydrophobic monomers in water. Copyright 2000 Academic Press.
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PMID:Microgel Particles as a Matrix for Polymerization: A Study of Poly(N-isopropylacrylamide)-Poly(N-methylpyrrole) Dispersions. 1065 27

Poly(vinyl acetate-co-ethylene) latex dispersions are prepared and their films investigated with a focus on the effect of composition upon redispersion. Films of dispersions containing sufficient amounts of poly(vinyl alcohol) (PVA) can be redispersed in water. This property is lost in the presence of surfactant, a fact which suggests a procedure to control film formation. It is demonstrated that redispersion is due to a PVA-membrane which separates the particles. Loss of redispersibility in the presence of surfactant proceeds with the breakup of the membranes and a corresponding change of film properties. Experimental data is provided by light microscopy, mechanical testing, and TEM in conjunction with a staining method new to the field. The hypothesis is developed that interaction with surfactant leads to imperfect PVA-membranes that are no longer able to prevent latex polymer interdiffusion. Fluorescence correlation spectroscopy demonstrates the formation of surfactant micelles, as well as the simultaneous adsorption and aggregation of PVA onto the micelles. It is concluded that the competing surface of the surfactant micelles traps enough PVA to cause thinning and fragmentation of the membranes surrounding the particles, which enables interdiffusion of latex polymer. This effect can be used to convert the system from one forming a redispersible coating to one forming a nonredispersible (permanent) film. Copyright 2000 Academic Press.
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PMID:Film Formation and Redispersion of Waterborne Latex Coatings. 1070 97

Poly(amidoamine)s (PAAs) are water-soluble polymers that display pH-dependent membrane activity. PAAs have the potential to act as a synthetic alternative to fusogenic peptides and thus promote endosomal escape. The purpose of this study was to investigate for the first time whether PAA have the ability to complex DNA, protect it from nuclease degradation and to promote transfection in vitro. PAAs ISA 1 (Mn 6900) and ISA 23 (Mn 10,500) and their 2-phenylethylamine containing analogues ISA 4 and ISA 22 (Mn approximately 8000) were studied. All PAAs retarded the electrophoretic mobility of lambda Hind III DNA demonstrating interpolyelectrolyte complex (IPEC) formation and toroids of 80-150 nm in diameter (10:1 polymer excess) were visible using TEM. DNase II inhibition was observed. At a polymer:DNA ratio of 10:1, this was ISA 1(89.6 +/- 6.1%), ISA 4 (92.2 +/- 11.2%), ISA 22 (69.4 +/- 3.7%), and ISA 23 (58.0 +/- 10.0%). PAAs demonstrated the ability to mediate pSV beta-galactosidase transfection of HepG2 cells. At a vector:DNA mass ratio of 5:1, ISA 23 showed equivalent transfection ability compared with polyethylenimine and LipofectIN and was more effective than LipofectACE. These properties suggest that PAAs warrant further development as endosomolytic vectors.
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PMID:Poly(amidoamine)s as potential nonviral vectors: ability to form interpolyelectrolyte complexes and to mediate transfection in vitro. 1171 5

Poly-N-methaniline/montmorillonite (PNMA-MMT) nanocomposite particles with high dielectric constant as well as suitable conductivity were synthesized by an emulsion intercalation method and characterized by FT-IR, XRD, and TEM spectrometry, respectively. The electrorheological (ER) properties of the suspensions of PNMA-MMT particles in silicone oil (20 wt%) were investigated under DC electric fields. It was found that the shear stress of poly-N-methaniline/montmorillonite electrorheological fluid (ERF) is 6.0 kPa in 3 kV/mm (74.5 s(-1)), which is 3.6 times that of electrorheological fluid at zero field, and also much higher than that of pure poly-N-methaniline (PNMA) and montmorillonite (MMT). In the range of 10-90 degrees C, the shear stress changes slightly with the temperature. The sedimentation ratio of PNMA-MMT ERF was about 97% after 60 days. Furthermore, the dielectric constant of PNMA-MMT nanocomposite was increased 3.74 times that of PNMA and 1.99 times that of MMT at 1000 Hz, the dielectric loss tangent also increased about 1.58 times that of PNMA. It is apparent that the notable ER effect of PNMA-MMT ER fluid was attributed to the prominent dielectric property of the poly-N-methaniline/montmorillonite nanocomposite particles.
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PMID:A new approach of enhancing the shear stress of electrorheological fluids of montmorillonite nanocomposite by emulsion intercalation of poly-N-methaniline. 1508 6

Poly(epsilon-caprolactone)-b-poly(ethylene glycol)-b-poly(epsilon-caprolactone) triblock copolymers were synthesized by the ring-opening polymerization of epsilon-caprolactone in the presence of hydroxyl-terminated poly(ethylene glycol) with different molecular weights, using stannous octoate catalyst. Micelles prepared by the precipitation method with these triblock copolymers exhibit a core-shell structure. The degradation behaviors of these core-shell micelles in aqueous solution were investigated by FT-IR, 1H NMR, GPC, DLS, TEM, and AFM. It was found that the degradation behavior of micelles in aqueous solution was quite different from that of bulk materials. The size of the micelles increased in the initial degradation stages and decreased gradually when the degradation period was extended. The caprolactone/ethylene oxide (CL/EO) ratio in micelles measured by NMR also shows an increase at the initial degradation stage and a decrease at later stages. The morphology of these micelles became more and more irregular during the degradation period. We explain the observed behavior by a two-stage degradation mechanism with interfacial erosion between the cores and the shells followed by core erosion.
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PMID:Degradation behavior of poly(epsilon-caprolactone)-b-poly(ethylene glycol)-b-poly(epsilon-caprolactone) micelles in aqueous solution. 1536 Feb 84

We demonstrate the use of aqueous-based layer-by-layer (LbL) processing and in situ nanoreactor chemistry for creating polyelectrolyte multilayer (PEM) nanocomposites in which the concentration of a nanoparticle species is modulated throughout the depth of the film in a complex way. Using the in situ growth of silver nanoparticles to selectively increase the refractive index of precisely defined regions of the PEM film, we have produced a digitized rugate filter, a special type of dielectric mirror possessing many thin layers that approximate a continuous, periodic refractive index profile. The design of the digital rugate from an initial smooth index profile was aided by a computer, and construction of the device was accomplished entirely by automated means. Poly(allylamine hydrochloride) (PAH) and poly(acrylic acid) (PAA) bilayer regions within the film supported subsequent nanoreactor chemistry and created the high refractive index regions of the film, while PAH and poly(styrenesulfonate) (PSS) bilayer regions remained unmodified and served as the low index regions. Spectroscopic results obtained from the assembled device confirm the presence of a reflection band that grows in amplitude with increasing silver incorporation, reaching a peak reflectance of 75% after five silver loading and exchange cycles. The rugate structure suppresses reflection sidebands, features common to Bragg reflectors and simpler dielectric mirror designs. Cross-sectional TEM reveals nanoscale control of the size and location of silver-containing domains within the PEM film. The ability of this method to control the placement of nanoparticles within PEMs enables the inexpensive, easy production of conformable optical coatings with tunable properties. In addition, these nanocomposite films may have applications in a number of other fields where precise control of effective gradients in physical properties throughout thin films has been shown to affect the overall performance in a desirable way.
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PMID:Creating effective refractive index gradients within polyelectrolyte multilayer films: molecularly assembled rugate filters. 1587 62

Magnetic multiwalled carbon nanotubes (MWNTs) were facilely prepared by the electrostatic self-assembly approach. Poly(2-diethylaminoethyl methacrylate) (PDEAEMA) was covalently grafted onto the surfaces of MWNTs by MWNT-initiated in situ atom transfer radical polymerization (ATRP) of 2-diethylaminoethyl methacrylate (DEAEMA). The PDEAEMA-grafted MWNTs were quaternized with methyl iodide (CH(3)I), resulting in cationic polyelectrolyte-grafted MWNTs (MWNT-PAmI). Magnetic iron oxide (Fe(3)O(4)) nanoparticles were loaded onto the MWNT surfaces by electrostatic self-assembling between MWNT-PAmI and Fe(3)O(4), affording magnetic nanotubes. The assembled capability of the nanoparticles can be adjusted to some extent by changing the feed ratio of Fe(3)O(4) to MWNT-PAmI. The obtained magnetic nanotubes were characterized with TEM, EDS, STEM, and element mapping analyses. TEM and EDS measurements confirmed the nanostructures and the components of the resulting nanoobjects. The magnetic nanotubes were assembled onto sheep red blood cells in a phosphate buffer solution, forming magnetic cells. The blood cells attached with or without magnetic nanotubes can be selectively manipulated in a magnetic field. These results promise a general and efficient strategy to magnetic nanotubes and the fascinating potential of such magnetic nanoobjects in applications of bionanoscience and technology.
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PMID:Magnetic carbon nanotubes: synthesis by electrostatic self-assembly approach and application in biomanipulations. 1659 89

Poly(amino acids) are natural chelating agents for various metal ions. Zinc ions were encapsulated in situ in a conductive polypyrrole film using polyglutamic acid as a localized complexing agent within the film. The subsequent electrochemical reduction of the metal ions to zero-valent metal leads to the formation of the nanoparticles. The electrochemical approach demonstrated in this report provides facile regeneration of the particles and also prevents aggregation of nanoparticles in the conductive polymeric film. The correlation of the amount of zinc with the thickness of the film indicates that the zinc resides largely in the outer layer of the film. TEM and EDS data show that the nanoparticles formed are composed of zinc and are 18 +/- 7 nm in diameter. The nanoparticle/ polymer composite was used to reduce halogenated organics, indicating its potential usefulness in remediation applications.
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PMID:Poly(amino acid)-facilitated electrochemical growth of metal nanoparticles. 1703 48

Poly(acrylic acid) (PAA) was polymerized on both termini of Pluronic F87 copolymer using the atom transfer radical polymerization technique to produce a novel block copolymer, PAA-b-F87-b-PAA (F87PAA). The loading of a cationic anticancer drug, doxorubicin (DOX), to F87PAA at different pH values was investigated using isothermal titration calorimetry (ITC), laser light scattering techniques, and UV-vis spectroscopy. At pH of 4.3-7.1, the ITC profile exhibited a significant exothermic peak, which indicated that the drug loading is an enthalpically driven process. At a pH of 4.3, the enthalpy maximum was significantly reduced in the presence of 2 M urea, indicating the existence of hydrogen bonds between the DOX and F87PAA copolymer. At a pH of 7.1, the fraction of bound DOX was close to the stoichiometric proportion of 1:1 to the molar concentration of carboxyl groups in the copolymer, where the drug loading is governed by electrostatic and stacking interactions. The TEM image of the complex indicated the formation of large compound micelles induced by the binding of DOX to the PAA segments.
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PMID:Synthesis and aggregation behavior of pluronic F87/poly(acrylic acid) block copolymer in the presence of doxorubicin. 1726 98


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