Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:6.5.1.2 (
DNA ligase
)
2,749
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The protozoan parasite
Entamoeba histolytica
is exposed to reactive oxygen and nitric oxide species that have the potential to damage its genome.
E. histolytica
harbors enzymes involved in DNA repair pathways like Base and Nucleotide Excision Repair. The majority of DNA repairs pathways converge in their final step in which a
DNA ligase
seals the DNA nicks. In contrast to other eukaryotes, the genome of
E. histolytica
encodes only one
DNA ligase
(EhDNAligI), suggesting that this ligase is involved in both DNA replication and DNA repair. Therefore, the aim of this work was to characterize EhDNAligI, its ligation fidelity and its ability to ligate opposite DNA mismatches and oxidative DNA lesions, and to study its expression changes and localization during and after recovery from UV and H
2
O
2
treatment. We found that EhDNAligI is a high-fidelity
DNA ligase
on canonical substrates and is able to discriminate erroneous base-pairing opposite DNA lesions. EhDNAligI expression decreases after DNA damage induced by UV and H
2
O
2
treatments, but it was upregulated during recovery time. Upon oxidative DNA damage, EhDNAligI relocates into the nucleus where it co-localizes with EhPCNA and the 8-oxoG adduct. The appearance and disappearance of 8-oxoG during and after both treatments suggest that DNA damaged was efficiently repaired because the mainly
NER
and BER components are expressed in this parasite and some of them were modulated after DNA insults. All these data disclose the relevance of EhDNAligI as a specialized and unique ligase in
E. histolytica
that may be involved in DNA repair of the 8-oxoG lesions.
...
PMID:The Sole DNA Ligase in
Entamoeba histolytica
Is a High-Fidelity DNA Ligase Involved in DNA Damage Repair. 3005 Aug 69
