Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:6.5.1.2 (
DNA ligase
)
2,749
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
In order to obtain the recombinant Bacillus subtilis strain, a transcriptional-translational control unit of the
alpha-amylase
gene of B. amyloliquefaciens was synthesized. The oligodeoxyribonucleotides were prepared by the modified triester method in solution and by the solid-phase approach. Then these oligonucleotides were joined by
DNA ligase
into two fragments which were cloned in the phage M13mp9 DNA and the plasmid pBR327. A plasmid harboring the site regulating the transcription of the
alpha-amylase
gene may be employed as vector for cloning the promoter-containing fragments in E. coli cells.
...
PMID:[Chemico-enzymatic synthesis of genetic elements for expression of synthetic genes in Bacillus subtilis cells]. 308 32
