Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:6.4.1.2 (acetyl-CoA carboxylase)
 2,876 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

3-Amino-1,2,4-triazole has been found to be an inhibitor of fatty acid synthesis by isolated rat hepatocytes. Half-maximal inhibition of fatty acid synthesis occurs at approximately 20mM. Acetyl-CoA carboxylase activity in homogenates of hepatocytes is not affected by previous exposure of the intact cells to 3-amino-1,2,4-triazole. The drug opposes the activation of partially purified acetyl-CoA carboxylase by citrate, but does not influence enzyme activity in the absence of citrate. As compared to fatty acid synthesis, cholesterol synthesis by the hepatocytes is more drastically depressed by incubation of the cells with 3-amino-1,2,4-triazole. Half-maximal inhibition of cholesterogenesis occurs at approximately 5 mM 8-amino-1,2,4-triazole.
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PMID:Inhibition of lipogenesis in isolated hepatocytes by 3-amino-1,2,4-triazole. 611 23

Acetyl-CoA carboxylase (ACCase) (EC.6.4.1.2) is an essential enzyme in fatty acid biosynthesis and, in world agriculture, commercial herbicides target this enzyme in plant species. In nearly all grass species the plastidic ACCase is strongly inhibited by commercial ACCase inhibiting herbicides [aryloxyphenoxypropionate (APP) and cyclohexanedione (CHD) herbicide chemicals]. Many ACCase herbicide resistant biotypes (populations) of L. rigidum have evolved, especially in Australia. In many cases, resistance to ACCase inhibiting herbicides is due to a resistant ACCase enzyme. Two ACCase herbicide resistant L. rigidum biotypes were studied to identify the molecular basis of ACCase inhibiting herbicide resistance. The carboxyl-transferase (CT) domain of the plastidic ACCase gene was amplified by PCR and sequenced. Amino acid substitutions in the CT domain were identified by comparison of sequences from resistant and susceptible plants. The amino acid residues Gln-102 (CAG codon) and Ile-127 (ATA codon) were substituted with a Glu residue (GAG codon) and Leu residue (TTA codon), respectively, in both resistant biotypes. Amino acid positions 102 and 127 within the fragment sequenced from L. rigidum corresponded to amino acid residues 1756 and 1781, respectively, in the A. myosuroides full ACCase sequence. Allele-specific PCR results further confirmed the mutations linked with resistance in these populations. The Ile-to-Leu substitution at position 1781 has been identified in other resistant grass species as endowing resistance to APP and CHD herbicides. The Gln-to-Glu substitution at position 1756 has not previously been reported and its role in herbicide resistance remains to be established.
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PMID:The molecular bases for resistance to acetyl co-enzyme A carboxylase (ACCase) inhibiting herbicides in two target-based resistant biotypes of annual ryegrass (Lolium rigidum). 1613 6