Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:6.4.1.2 (
acetyl-CoA carboxylase
)
2,876
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
This study investigated the chemical and genetic mechanisms of anaerobic fermentation of waste activated sludge (WAS) with
CaO
2
addition at ambient temperature. The microbial community structures, key microorganisms, functional profiles and related carbohydrate-active enzymes were further revealed according to metagenomic sequencing combining with 16S rRNA gene amplicon sequencing. Results showed that the prolonged period of alkaline condition generated from
CaO
2
contributed significantly to the continuous destruction of WAS, and the oxidative environment caused by
CaO
2
further enhanced flocs dissolution. This synergistic effect also significantly changed the microbial community. Oxidation contributed more than the alkaline condition to the decline of microbial diversity, while the effect of alkaline condition was greater than that of oxidation in the change of microbial community structure. The key enhanced genes associated with fatty acid biosynthesis pathways with
CaO
2
addition were highlighted. Three kinds of high-abundance
acetyl-CoA carboxylase
genes and eleven kinds of synthetase, hydrolase, lyase and oxidoreductase genes promoted by
CaO
2
were distributed throughout each branch of fatty acid biosynthesis pathway (ko00061). Moreover, carbohydrate binding modules (CBMs) and glycoside hydrolases (GHs) were the top two carbohydrate-active enzymes (CAZymes) improved by
CaO
2
addition.
CaO
2
can also effectively promote the function of lysozyme and the metabolism of several monosaccharides. This work provides a deep insight into the advantage of
CaO
2
in promoting sludge solubilization and acidification at the genetic levels, thus expanding the application of
CaO
2
in sludge treatment and resource recovery.
...
PMID:Metagenomic characterization of the enhanced performance of anaerobic fermentation of waste activated sludge with CaO
2
addition at ambient temperature: Fatty acid biosynthesis metabolic pathway and CAZymes. 3181 14
