Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:6.4.1.2 (
acetyl-CoA carboxylase
)
2,876
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Messenger RNA from Candida lipolytica directs the synthesis of complete acetyl-coenzyme-A carboxylase in the mRNA-dependent reticulocyte lysate cell-free translation system. The identity of the translation product is evidenced by the following results: first, it is immunoprecipitated with antibody to
acetyl-CoA carboxylase
and competes with authentic
acetyl-CoA carboxylase
for binding to the antibody; secondly, it co-migrates with authentic
acetyl-CoA carboxylase
(Mr 230000) upon dodecylsulfate/polyacrylamide gel electrophoresis; finally, the peptide fragments formed by its partial proteolysis with
papain
or alpha-chymotrypsin are identical with those formed from authentic
acetyl-CoA carboxylase
. With the use of this assay system, it has been demonstrated that the level of
acetyl-CoA carboxylase
mRNA activity in C. lipolytica cells decreases with increasing concentrations of oleic acid in culture medium and that the changes in the mRNA activity parallel those in the cellular level of
acetyl-CoA carboxylase
. This finding, in conjunction with our previous study, indicates that the diminished synthesis of
acetyl-CoA carboxylase
in cells grown in the presence of fatty acid is due to a reduced level of the mRNA coding for the enzyme.
...
PMID:Cell-free translation and regulation of Candida lipolytica acetyl-coenzyme-A carboxylase messenger RNA. 610 7
