Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:6.4.1.2 (
acetyl-CoA carboxylase
)
2,876
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The activity of
acetyl-CoA carboxylase
(
ACC
), the rate-limiting enzyme of fatty acid biosynthesis, can be regulated by both adenine and guanine nucleotides in vitro. We have employed two inhibitors of
IMP dehydrogenase
, ribavarin and tiazofurin, to investigate a possible role for intracellular nucleotides in
ACC
regulation in rat adipocytes. Ribavarin, but not tiazofurin, leads to a profound time-dependent inhibition of
ACC
activity that is associated with a decrease in both intracellular ATP and GTP. This inactivating effect is largely reversed with guanosine, accompanied by increases in both ATP and GTP levels. Epinephrine-mediated inactivation of
ACC
in intact cells is not altered by ribavarin incubation. However, in these experiments, insulin-mediated activation is observed only after ribavarin-induced inhibition of the enzyme. These data suggest that nucleotides may modulate
ACC
activity and influence is regulation by insulin in intact cells. The possible mechanisms underlying the insulin activation of
ACC
and the role of intracellular nucleotides in insulin action are discussed.
...
PMID:Modulation of acetyl-CoA carboxylase by inhibitors of IMP dehydrogenase: implications for insulin regulation. 288 May 60
