Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
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Drug
Enzyme
Compound
Query: EC:6.4.1.2 (
acetyl-CoA carboxylase
)
2,876
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The
acetyl-CoA carboxylase
(
ACC
) gene contains two distinct promoters, denoted PI and PII. PI is responsible for the generation of class I
ACC
mRNAs which are induced in a tissue-specific manner under lipogenic conditions. PII generates class II
ACC
mRNAs which are expressed constitutively. During 30A5 preadipocyte differentiation, both promoters are activated; the preadipocytes must be pretreated with cAMP for this activation to occur. In this report, we present evidence that CAAT enhancer-binding protein-beta (
C/EBP-beta
) is induced and involved in the PI activation by cAMP. Expression of the reporter gene under the control of the PI promoter is activated within 3 h after treatment of 30A5 cells with a cyclic AMP analogue, 8-(4-chlorophenylthio)-adenosine 3',5'-cyclic monophosphate, and 3-isobutyl-1-methylxanthine, in association with the accumulation of
C/EBP-beta
mRNA and protein. These accumulations were inhibited in the presence of H8, a protein kinase inhibitor; H8 also inhibited activation of PI by cAMP. However, the induction of reporter gene expression and the increase of
C/EBP-beta
mRNA by cAMP were not affected by treatment with tumor necrosis factor alpha, which completely inhibited the accumulation of C/EBP-alpha mRNA. Overexpression of
C/EBP-beta
by transfection with the
C/EBP-beta
gene led to increased binding of
C/EBP-beta
to DNA and partial PI activation. cAMP did not affect the amount of
C/EBP-beta
binding to the DNA but did promote phosphorylation of
C/EBP-beta
and PI activation. As in the case of C/EBP-alpha,
C/EBP-beta
bound to the CCAAT box of the PI promoter. These results indicate that cAMP not only induces, but also activates, bound
C/EBP-beta
through phosphorylation for PI activation. Our studies also indicate that cAMP induces C/EBP-alpha.
C/EBP-beta
induction, however, precedes that of C/EBP-alpha.
...
PMID:cAMP activation of CAAT enhancer-binding protein-beta gene expression and promoter I of acetyl-CoA carboxylase. 754 64
