Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:6.4.1.1 (
pyruvate carboxylase
)
1,516
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The importance of H
2
S in biology and medicine has been widely recognized in recent years, and protein
S-
sulfhydration is proposed to mediate the direct actions of H
2
S bioactivity in the body.
Thioredoxin
1 (Trx1) is an important reducing enzyme that cleaves disulfides in proteins and acts as an
S
-denitrosylase. The regulation of Trx1 on protein
S
-sulfhydration is unclear. Here we showed that Trx1 facilitates protein
S
-desulfhydration. Overexpression of Trx1 attenuated the basal level and H
2
S-induced protein
S
-sulfhydration by direct interaction with
S
-sulfhydrated proteins, i.e., glyceraldehyde 3-phosphate dehydrogenase and
pyruvate carboxylase
. In contrast, knockdown of Trx1 mRNA expression by short interfering RNA or blockage of Trx1 redox activity with PX12 or 2,4-dinitrochlorobenzene enhanced protein
S
-sulfhydration. Mutation of cysteine-32 but not cysteine-35 in the Trp-Cys
32
-Gly-Pro-Cys
35
motif eliminated the binding of Trx1 with
S
-sulfhydrated proteins and abolished the
S
-desulfhydrating effect of Trx1. All these data suggest that Trx1 acts as an
S
-desulfhydrase.
...
PMID:Thioredoxin 1 regulation of protein
S
-desulfhydration. 2895 4
