Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:6.4.1.1 (pyruvate carboxylase)
 1,516 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

An oligodendrocyte-enriched culture was incubated with the antidepressant drugs, amitriptyline, desipramine, mianserin and citalopram, in a concentration of 3 X 10(-6) M for 24 h and 14 days. The activity of glycerol-3-phosphate dehydrogenase (G3PDH), lactate dehydrogenase (LDH), pyruvate carboxylase (PC) and the incorporation of [3H]thymidine was measured. An increase in both the incorporation of [3H]thymidine into DNA and the activities of G3PDH and PC was observed. However, LDH activity decreased after a 2-week incubation. The above results indicate that the antidepressant drugs change the metabolism of glial cells.
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PMID:Effects of some antidepressant drugs on the activity of glial cell enzymes in culture. 272 55

This study aimed to test the hypothesis that adipocyte TG accumulation could be altered by specifically perturbing pyruvate metabolism. We treated cultured 3T3-L1 adipocytes with chemical inhibitors of lactate dehydrogenase (LDH) and pyruvate carboxylase (PC), and characterized their global effects on intermediary metabolism using metabolic flux and isotopomer analysis. Inhibiting the enzymes over several days did not alter the adipocyte differentiation program as assessed by the expression levels of peroxisome proliferator-activated receptor-gamma and glycerol-3-phosphate dehydrogenase. The main metabolic effects were to up-regulate intracellular lipolysis and decrease TG accumulation. Inhibiting PC also up-regulated glycolysis. Flux estimates indicated that the reduction in TG was due to decreased de novo fatty acid synthesis. Exogenous addition of free fatty acids dose-dependently increased the cellular TG level in the inhibitor-treated adipocytes, but not in untreated control cells. The results of this study support our hypothesis regarding the critical role of pyruvate reactions in TG synthesis.
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PMID:Impact of perturbed pyruvate metabolism on adipocyte triglyceride accumulation. 1968 93