Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:6.3.5.5 (CPS)
 1,262 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Recently, the CPS biosynthetic loci for several strains of Campylobacter jejuni were sequenced and revealed evidence for multiple mechanisms of structural variation. In this study, the CPS structure for the HS:1 serostrain of C. jejuni was determined using mass spectrometry and NMR at 600 MHz equipped with an ultra-sensitive cryogenically cooled probe. Analysis of CPS purified using a mild enzymatic method revealed a teichoic acid-like [-4)-alpha-d-Galp-(1-2)-(R)-Gro-(1-P](n), repeating unit, where Gro is glycerol. Two branches at C-2 and C-3 of galactose were identified as beta-d-fructofuranoses substituted at C-3 with CH(3)OP(O)(NH(2))(OR) groups. Structural heterogeneity was due to nonstoichiometric glycosylation at C-3 of galactose and variable phosphoramidate groups. Identical structural features were found for cell-bound CPS on intact cells using proton homonuclear and (31)P heteronuclear two-dimensional HR-MAS NMR at 500 MHz. In contrast, spectroscopic data acquired for hot water/phenol purified CPS was complicated by the hydrolysis and subsequent loss of labile groups during extraction. Collectively, the results of this study established the importance of using sensitive isolation techniques and HR-MAS NMR to examine CPS structures in vivo when labile groups are present. This study uncovered how incorporation of variable O-methyl phosphoramidate groups on nonstoichiometric fructose branches is used in C. jejuni HS:1 as a strategy to produce a highly complex polysaccharide from its small CPS biosynthetic locus and a limited number of sugars.
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PMID:The HS:1 serostrain of Campylobacter jejuni has a complex teichoic acid-like capsular polysaccharide with nonstoichiometric fructofuranose branches and O-methyl phosphoramidate groups. 1612 10

We report the evidence for the cellular uptake of gold nanoparticles via the phagocytosis mechanism in murine macrophage cells strongly supported by TEM and optical microscopy. Nanoparticles were prepared using several biocompatible molecules of choice (5-aminovaleric acid, l-DOPA, melatonin, and serotonin hydrochloride) as stabilizers for gold colloids. Their surface chemistry was fully characterized by UV-vis, ATR-FTIR, (1)H NMR, and HR-MAS (1)H NMR spectroscopies, and size distribution was determined by CPS disc centrifuge and TEM. Differences in coatings were evaluated against cellular uptake, and a preferential movement of macrophages toward 5-aminovaleric acid-modified gold nanoparticles was shown, leading to the fast accumulation of nanoparticles in the cytosol.
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PMID:Phagocytosis of biocompatible gold nanoparticles. 2079 74

Creativity is essential for success in business, especially in the contemporary world where innovations are a cornerstone of competitive advantage. A large body of literature has examined many factors which influence creative abilities with the work environment being the factor of increasing importance. Creativity has been associated with the work environment through a number of theoretical frameworks and empirical studies. However, the relationship between the work environment and creativity in different cultures has not been explored sufficiently. This paper addresses this gap in the literature by linking the concept of a creativity-supporting work environment and culture. To that end, elements of the work environment (physical, socio-organisational, and fun in the workplace) and creative personality have been analysed on multinational samples. The results clearly point that the relationship exists between dimensions of the national culture (individualism, uncertainty avoidance, masculinity, and power distance) and specific elements of a creativity-supporting work environment. Practitioner summary: Companies experience high pressure for generating creative ideas. As a result, stimulating employees' creativity has become a key strategic aim. This study, through cross-cultural primary research, provides clear managerial implications and practical advice for successful implementation and quality adoption of the creativity-supporting work environment worldwide. Abbreviations: A-FOs: activity-based flexible offices; AVG: average; CDQS: creativity development quick scan; CP: creative personality; CPS: creative personality scale; FWP: fun in the workplace; HF/E: human factors and ergonomics; IDV: individualism; MAS: masculinity; MBA: Master of Business Administration; PDI: power distance; PWE: physical work environment; SWE: socio-organisational work environment; UAI: uncertainty avoidance; USA: United States of America; WE: work environment.
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PMID:The influence of cultural dimensions when implementing fun in the workplace approach as a driver of creativity. 3103 99