Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:6.3.5.5 (
CPS
)
1,262
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Marine vibrios,
Vibrio parahaemolyticus
,
V. vulnificus
and
V. cholerae
are responsible of the majority of food-borne human infections by consumption of bivalve shellfish. The aim of the present study was to ascertain the occurrence of these bacteria, and their potential pathogenicity, in the Manila clam
R. philippinarum
from Emilia Romagna (ER) and Sardinia (SR) regions, Italy. Isolation was performed on CHROMagar
TM
vibrio with subculture on (thiosulfate-citrate-bile salts-sucrose) Agar and m-modified-cellobiose-polymyxin b-colistin (-CPC) Agar. Suspected strains were purified, biochemically characterized and genotyped by simplex polymerase chain reaction (PCR) for the specie-specific and pathogenic gene markers:
V. parahaemolyticus
(
toxR
P,
tdh
and
trh
);
V
.
vulnificus
(
vvhA
,
hsp
,
vcg
C,
vcg
E,
CPS
operon allele 1,
CPS
operon
allele 2
, 16s-rRNA operon allele A, 16s-rRNA operon allele B;
V. cholerae
(
toxR
C,
hlya
,
tcpI
,
tcpA
,
ctxA
,
ctxB
,
stn
/
sto
). Moreover a multiplex PCR was applied to the SR bivalve shellfish, for the simultaneous detection of the three targets directly on homogenate samples, targeting the species-specific gene for
V. cholerae
(
toxR
C),
V. parahaemolyticus
(
toxR
P) and
V. vulnificus
(
vvhA
). As a result of phenotyping and genotyping of isolates, bivalve shellfish from ER resulted positive for
V
.
parahaemolyticus
(27.8%) and
V. vulnificus
(10.1%), but negative for
V. cholerae
. Shellfish from SR resulted positive for
V
.
parahaemolyticus
(30.3%),
V. vulnificus
(6.1%) and
V. cholerae
(3%). No significant differences emerged between the two areas (P>0.05).
...
PMID:Occurrence of Vibrio
Parahaemolyticus
, Vibrio
Cholerae
and
Vibrio Vulnificus
in the Clam
Ruditapes Philippinarum
(Adams & Reeve, 1850) from Emilia Romagna and Sardinia, Italy. 2780 Apr 36
