Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:6.3.4.6 (urease)
 7,490 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Physiological properties and proportion of typical features of Pityrosporum pachydermatis were determined on 385 strains from clinical cases of O.E and dermatitis in dogs. Carbohydrates and nitrogen assimilation were determined auxanographically. Urease production and enzyme release were assessed on Christensen's medium and API-ZYM respectively. All strains oxidised carbohydrates in the OF test. 90% assimilation of glucose and production of urease are typical of Pityrosporum, contrary to 100% positive reactions in literature data. Production of acid and alkaline phosphatases, phosphohydrolase, leucin arylamidase, and beta-glucosidase dominated, while lipase C14, esterase-lipase C8, esterase C14 and alpha-galactosidase were variable.
Arch Vet Pol 1994
PMID:Evaluation of selected physiological and morphological characteristics of Pityrosporum pachydermatis isolated from clinical cases of otitis externa and dermatitis in dogs and cats. 889 Nov 71

The presence of Helicobacter pylori in the gastric mucosa of 150 patients with gastric, duodenal ulcer and endoscopic gastritis was investigated by rapid urease-based test, culture, histological examination and serology. Overall, 78% were positive for Helicobacter pylori by rapid urease-based test, 69.3% by culture, 53.7% by histological staining and 93.2% by serology (p < 0.001). The frequency of Helicobacter pylori was higher in patients with gastric or duodenal ulcer compared with those with endoscopic gastritis and those after gastric resection. However, the differences were significant only with reference to rapid urease-based test, (p < 0.02). The EIA (Roche) serological test had the highest sensitivity and the lowest specificity. This indicates the seed for serological kit to be independently evaluated on the population to be studied. The rapid urease-based test was simple, rapid and inexpensive, and it was more sensitive and equally specific compared with culture and Giemsa stain.
Pol Tyg Lek 1996 Apr
PMID:[Urease test, microbiologic tests, histologic and serologic tests for the evaluation of Helicobacter pylori infections in persons with peptic ulcer and gastritis]. 896 63

They examined 114 young health volunteers to establish a frequency of inflammation of gastric mucous membrane and(or) duodenal bulbous, a frequency of Helicobacter pylori infection and interdependence between infection and inflammation, smoking and nourishment. They evaluated a frequency and intensity of inflammation in antrum, fundus and duodenal bulbus and present of Helicobacter pylori using an urease test and microscopic examination. They noted in young inhabitants of Warsaw, appearance of asymptomatic gastritis in more than 53% cases, an inflammation of duodenal bulbus in 34% and H.pylori infection in 50% cases.
Pol Merkur Lekarski 1996 Sep
PMID:[Changes in gastric mucosa and Helicobacter pylori infection in young health volunteers]. 913 80

In the present study, the effectiveness of a triple therapy for eradication of Helicobacter pylori was evaluated. Therapy consisted of 120 mg tripotassium dicitrato bismuthate q.d.s. for four weeks, 500 mg amoxycillin q.d.s. and 500 mg metronidazole t.d.s. for two weeks. In 77 Helicobacter pylori-positive patients with duodenal ulcers (n = 32), gastritis (n = 18) and after gastric resection (n = 7), rapid urease-based test, culture, histology and serology were used to confirm the eradication, or relapse. The overall eradication rate was 75.3%, ulcers were healed in 82.1% and an improvement of the endoscopic gastritis was observed in 75.3% of the patients. The eradication rates were not statistically different among the subgroups. 1-3 months after the treatment IgG titres had fallen by 25% and over in 67.5% of the patients irrespective of the success of bacterial eradication. Side effects, particularly diarrhoea and nausea, were common (53.2%) but mild. Only 6.5% of the patients' had to discontinue the treatment because side effects became intolerable.
Pol Merkur Lekarski 1996 Sep
PMID:[Early results of treating Helicobacter pylori infections in patients with gastric ulcer and gastritis]. 913 81

The effect of concentration of acetohydroxamic acid (AHA) on inhibition of jack bean urease in phosphate buffer, pH 7.0, at 25 degrees C, was studied. The measurements were performed at urease concentration of 2.5 mg/100 cm3 for concentrations of urea and AHA ranging in the range of 2-50 mmol dm-3 and 0.25-10 mmol dm-3, respectively. The reactions were monitored by two techniques: analytical and enthalpimetric. For the analytical technique the growth of ammonia concentration in the course of the reaction was determined. From the recorded progress curves the following parameters were calculated for each inhibitor concentration: the initial reaction rate, the steady-state rate and the inversion constant. From these parameters the inhibition constants of the initial and steady-state stages of the reaction, Ki and Ki, were calculated. The former constant did not change whereas the latter one proved to decrease quickly with an increase in inhibitor concentration. This behaviour resulted from the fact that the inactive complex EI was not a product of internal inversion but was formed in the reaction: 2/3I + EI-->(EI.2/3I). The dissociation constant of this complex is equal to about 0.3 x 10(-3) (mol dm-3)2/3.
Acta Biochim Pol 1997
PMID:The enthalpimetric determination of inhibition constants for the inhibition of urease by acetohydroxamic acid. 924 59

Three hundred seventy four patients with duodenal ulcers and Helicobacter pylori infections were given a four-week treatment of bismuth or ranitidin. In all patients two-week antibiotic therapy were given. Endoscopies with urease tests and histologic examinations were performed before initiation and four weeks after cessation of therapy. Four-week therapy with ranitidini and two-week therapy with amoxicillin and metronidasole is highly effective (89.6%) in duodenal ulcer healing and symptom improvement comparison to bismuth and antibiotic therapy.
Pol Merkur Lekarski 1998 Aug
PMID:[The treatment of chronic duodenal ulcer with accompanying Helicobacter pylori infection]. 1010 57

Recurrent formation of renal calculi may be prevented by selective medical therapy designed to correct underlying metabolic disturbances in urine biochemistry. The aim of the study was to estimate potential risk factors of urolithiasis in 102 children before ESWL treatment. Metabolic disturbances followed by anatomical malformations of urinary tract, recurrent urinary tract infections caused by urease-induced microorganisms are the most frequent risk factors. In single cases possible risk factors were: immobilization and chronic corticosteroid therapy.
Pol Merkur Lekarski 2000 Apr
PMID:[Etiology of nephrolithiasis in children: own observation]. 1089 98

Both urea and urease were subjects of early scientific investigations. Urea was the first organic molecule to be synthesized and jack bean urease was the first enzyme ever to be crystallized. About 50 years later it was shown to be the first nickel metalloenzyme. Since then, nickel-dependent ureases have been isolated from many bacteria, fungi and higher plants. They have similar structures and mechanisms of catalysis. A urease apoenzyme needs to be activated. This process requires participation of several accessory proteins that incorporate nickel into the urease forming catalytic site. In this review, ureases from various organisms are briefly described and the similarities of their structures discussed. Moreover, the significance of urea recycling in plants is explained and recent literature data about the function and activation of plant ureases are presented.
Acta Biochim Pol 2000
PMID:Plant ureases: roles and regulation. 1199 9

The aim of this study was the evaluation of the occurrence of Helicobacter-like organisms in the gastric mucosa of dogs. The study was performed on samples of gastric mucosa obtained during necropsies from 94 dogs euthanasized for various reasons. Histopathological examination and also urease test was performed. Helicobacter sp. was found in 85.10% of examined dogs. In 36% cases the presence of bacteria was accompanied by chronic inflammation of gastric mucosa localized mainly in pyloric region. In 97.59% samples positive results of urease test were associated with histopathological confirmation of gastric Helicobacter-like organisms.
Pol J Vet Sci 2003
PMID:The presence of Helicobacter-like microorganisms in the gastric mucosa in dogs. 1470 68

Uraemic patients frequently complain of gastrointestinal ailments. There are a lot of factors responsible for the occurrence of these symptoms. It is considered--among other things--that high level of urea in gastric juice and hypergastrinaemia contribute to the damage of stomach and duodenum mucosa. Does H. pylori infection also produce pathological changes within upper gastrointestinal tract in these patients? The aim of the study was to determine the prevalence of H. pylori infection and macroscopic and microscopic estimation of oesophagus, stomach and duodenum mucosa in haemodialysis patients and renal transplant recipients. A total of 39 patients were taken under investigation: 27 among them were treated with haemodialyses and 12 were after kidney transplantation. In all patients upper gastrointestinal endoscopies were performed with collection of biopsy specimens for histological analysis and a urease test. Serological examinations were carried out in order to detect anti-CagA H. pylori antibody. In summary, we found that prevalence of H. pylori infection in uremic patients on chronic haemodialysis and renal transplant recipients was significantly lower than that in patients with normal renal function. This may be a consequence of medication and/or protection by a high urea concentration. Elevated blood urea seems to correlate with a high prevalence of gastroduodenal mucosal lesions.
Pol Arch Med Wewn 2004 Mar
PMID:[Is it necessary to treat Helicobacter pylori infection in patients with end-stage renal failure and in renal transplant recipients?]. 1523 Feb 10


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