Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:6.3.4.6 (urease)
 7,490 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

By using nested polymerase chain reaction (PCR) composed of three primers that derived from Helicobacter pylori's (Hp) urease gene region, we have not only successfully amplified control Hp and 214 clinic samples but also studied deeply preparation of the sample, composition of PCR reaction liquid and circulatory mental conditions of the PCR. So this method shortens the operation time, simplifies processes and reduces costs. Compared with urease test (54%) and Warthin-Starry silver stained test (50%), ever used the positive rate of PCR is the highest (61%), so our method is more specific, sensitive, simple, rapid and accurate, and could be applied to the routine clinical detection.
Zhonghua Nei Ke Za Zhi 1995 Apr
PMID:[Amplifying Helicobacter pylori's urease gene by nested polymerase chain reaction]. 758 3

Helicobacter pylori (Hp) were detected in the dental plaque in 40 patients with peptic ulcer, chronic gastritis or gastric cancer with rapid urease test, anti-Hp fluorescein-labelled antibody staining, bacterial culture and electronic microscopy. At the same time, biopsy specimens from the gastric antrum of these patients were studied with WS staining and rapid urease test to detect Hp. The results show that a great lot of Hps is present in most of the patients' dental plaque. The morphological, biochemical and immunological characteristics of the Hp in the dental plaque are similar to those Hp in the gastric mucosa. The above results reveal that the Hp in the dental plaque may be identical with those in the stomach.
Zhonghua Nei Ke Za Zhi 1994 Jul
PMID:[Helicobacter pylori in the dental plaque]. 786 41

Depending on the character for the Helicobacter pylori (HP) to decompose the urea by its endogenous urease, we have investigated the possibility to diagnose HP infection by 13C-urea breath test in 55 cases. Also, we compared the diagnostic results from 13C-urea breath test with those from culture, histology, and rapid urease test. The satisfied accuracy for the detection of HP infection with 13C-urea breath test was indicated.
Zhonghua Nei Ke Za Zhi 1993 Mar
PMID:[Diagnosis of Helicobacter pylori infection by 13C-urea breath test]. 822 81

A sensitive and specific serological diagnostic test for Helicobacter pylori infection has been developed and validated in 120 patients with dyspeptic symptoms undergoing endoscopy. This test is to use urease, a protein unique to H. pylori, as the basis for the enzyme linked immunosorbent assay (ELISA) that detects serum H. pylori urease antibodies. The ELISA mean optical density (OD) in H. pylori-positive group is higher than that in H. pylori-negative group (0.57 +/- 0.23 vs 0.24 +/- 0.15, P < 0.001), a cut-off 0.3 OD yields a sensitivity of 95% and a specificity of 93%. Serum absorption test showed that Escherichia coli, Klebsiella pneumonia, Proteus mirabilis, Yersinia enterocolotica, Pseudomonas aeruginosa cell lysate do not influence serum H. pylori urease antibody level, though they all have urease except E. coli. The result implied that H. pylori urease can be a good antigen to detect serum H. pylori antibody and it would be useful for epidemiological survey and routine diagnostic approach. Nearly half of the blood donors showed positive result with H. pylori urease antibody. It is suggested that H. pylori infection is quite common in the asymptomatic population.
Zhonghua Nei Ke Za Zhi 1993 May
PMID:[An urease enzyme linked immunosorbent assay for detection of Helicobacter pylori infection]. 826 56

The diagnostic accuracy of gastric urease activity for Helicobacter pylori (HP) infection were studied in 98 patients with peptic ulcer, gastritis and chronic renal failure. Gastric aspirates were analyzed for urea nitrogen and ammonia. Urease activity was calculated as the ratio of gastric ammonia to sum of gastric urea nitrogen and ammonia. Gold standard assessments to HP infection were rapid urease test, histology and/or culture. The results showed that the values of urease activity in patients with HP infection higher than that in patients without HP infection. This were further assessed by eradication of HP. Sixteen patients with HP infection were treated with DeNol 330 mg/day for 4 weeks. HP was eradicated in 8/16 patients. In those cases urease activity reduced (P < 0.05). If taken 0.31 as the cut-off index of urease activity to diagnosis HP infection, the sensitivity, specificity and accuracy were 95%, 84%, 93% respectively and were not affected by blood urea nitrogen.
Zhonghua Nei Ke Za Zhi 1993 Jan
PMID:[The diagnostic accuracy of gastric urease activity for Helicobacter pylori]. 840 18

Serological testing has recently been proposed as an aid in diagnosis of H. pylori infections. In this study, an immunoblot method has been evaluated to diagnose H. pylori infection serologically by comparing 104 serum specimens from patients with a positive Gram stain and/or culture result and a positive urease test on biopsy material, as well as 30 serum specimens from patients with negative urease test, and negative microscopy and culture results. Thirteen bands could be identified by immunoblotting and were found significantly more frequent in patients with H. pylori infections than in patients without such infections. A semiquantitative grading system was introduced with score 1 for each reactive band and score 1/2 for a weakly reactive band. If score 2 was the cut-off value for IgG antibody, then the sensitivity, specificity, positive predictive value and negative predictive value of the immunoblot test were 100.0%, 90.0%, 97.2% and 100.0% respectively. There was no marked cross reaction with both Campylobacter jejuni and Campylobacter coli.
Zhonghua Nei Ke Za Zhi 1996 Jan
PMID:[Serodiagnosis of Helicobacter pylori infections by detection of immunoglobulin G antibodies with an immunoblot technique]. 927 37

The aim of this study is to determine the eradication rate in Helicobacter pylori (Hp) infection using omeprazole 20 mg twice daily, clarithromycin 500 mg twice daily and amoxicillin 1,000 mg twice daily for one or two weeks, in comparison with colloidal bismuth subcitrate 240 mg twice daily, amoxicillin 1,000 mg twice daily and metronidazole 400 mg twice daily for two weeks. 141 patients with Hp associated chronic gastritis or duodenal ulcer were randomly divided into 3 groups. Group I (n = 48) treated with triple therapy of omeprazole, clarithromycin and amoxicillin for 1 week. Group II (n = 47) treated with the same drug and dosage for 2 weeks. Group III (n = 46) treated with triple therapy of colloidal bismuth subcitrate, amoxicillin and metronidazole for 2 weeks. The Hp status was determined by rapid urease test and histology of Warthin-Starry silver stain. The eradication rates of Group I, Group II and Group III were 89.6%, 95.7% and 71.7% respectively. The difference between Group I and Group III, or Group II and Group III was significant. The difference of eradication rate between Group I and Group II was statistically insignificant. The frequency of side effects in Group III was higher than that in Group I or Group II. Although 21.1% of the patients in Group I and II experienced a metallic taste, none discontinued the medication. Our results show that triple therapy with omeprazole, clarithromycin and amoxicillin is an effective and well tolerated treatment for eradication of Hp infection and may be an alternative regimen to the old standard triple therapy.
Zhonghua Nei Ke Za Zhi 1996 Dec
PMID:[Omeprazole, clarithromycin and amoxicillin therapy for Helicobacter pylori infection]. 959 3