Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:6.3.4.6 (
urease
)
7,490
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The urocanic acid cis isomer (cis-
UCA
) is a possible cutaneous photoreceptor for the immunomodulatory phenomena that follow ultraviolet B irradiation. Several experiments in animals show an inhibitory action of cis-
UCA
on cellular immunity. However, the action of cis-
UCA
on the synthesis of cytokines in keratinocytes remains unknown. Long-term cultures of normal human keratiocytes were prepared in a serum-free medium, and stimulated with 1 microgram/ml of phorbol 12-myristate 13-acetate (TPA) and
UCA
or UVB-
UCA
(10-100 micrograms/ml). Synthesis of the following cytokines was measured using ELISA and Northern blot techniques: TNF-alpha, IL-1 alpha, IL-1 beta, IL-6, IL-8 and
TGF-beta
1. TPA increased TNF-alpha protein levels in culture supernatants. No changes in Il-1 alpha and IL-1 beta protein levels were detected in basal culture supernatant after TPA stimulus. TPA augmented RNA expression for TNF-alpha, IL-1 alpha, IL-1 beta and
TGF-beta
1.
UCA
isomers did not induce cytokine changes in protein synthesis. Expression of IL-1 alpha and IL-1 beta genes was increased after exposure to 100 micrograms/ml UVB-
UCA
(70 micrograms/ml cis-
UCA
). A slight increase in TNF-alpha RNA expression was detected when the dose of UVB-
UCA
reached 100 micrograms/ml. No effects on cytokine synthesis were found after
UCA
stimulus. These results suggest that low doses of cis-
UCA
do not effect cytokine synthesis by keratinocytes.
...
PMID:Effects of low concentrations of cis- and trans-urocanic acid on cytokine elaboration by keratinocytes. 918 8
