Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:6.3.4.6 (urease)
 7,490 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The material consisted of samples of mucous membrane of stomach and duodenum obtained during endoscopy in patients with clinical symptoms of peptic ulcer of stomach or the duodenum or with dyspeptic problems. Samples were tested for presence of H. pylori by culture on brain-heart agar supplemented with 7% of horse blood. Direct test for urease production was also performed. Isolated strains were identified basing on morphology of growth, Gram-stained preparation, mobility of microorganism, production of oxidase, catalase and urease, and ability to agglutinate in immune goat serum for standard H. pylori strain. Out of tested 217 samples, positive result was obtained in 141 cases. Urease test was positive in 138 cases. Isolated strains were tested for susceptibility to 14 antimicrobials. They were all resistant to nalidixic acid and susceptible in 90-100% to cephalothin, furazolidone, gentamicin and ofloxacin.
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PMID:[Helicobacter pylori in mucous membrane of stomach and duodenum of patients with symptoms of ulceration and dyspepsia]. 818 27

Forty-two gastric biopsies, in two transport media, were homogenized and cultured on three media under micro-aerophilic conditions. Brain-heart infusion agar with a commercial antibiotic supplement (giving 10 mg vancomycin, 5 mg trimethoprim and 2500 i.u. polymixin per litre) yielded the best results. Ordinary chocolate (heated) human blood agar could be used in laboratories with limited resources. Growth was obtained in 4-6 days at 37 degrees C. All isolates were sensitive to metronidazole. The resistance to nalidixic acid and rapid urease production of Helicobacter pylori could be used to differentiate this species from Campylobacter spp. Indicator medium (brain-heart infusion broth with 7% human blood and 40 mg 2,3,5-triphenyletetrazolium chloride agar/litre) also proved useful in identification.
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PMID:Helicobacter pylori: isolation from gastric biopsy specimens. 831 73

The susceptibility of Helicobacter pylori to the antimicrobial system involving lactoperoxidase, hydrogen peroxide and thiocyanate was investigated. The inhibitory effect of the system on the urease activity of H. pylori, which plays a role in its colonisation of the stomach, was also investigated. Twelve H. pylori strains examined, including 10 clinical isolates, were all inhibited by the peroxidase system in brain-heart infusion broth supplemented with fetal calf serum, but to different extents. The killing effect was observed within 3 h. Although bacterial viability recovered afterwards, there was still a clear difference between cultures incubated in the presence of the complete system and control cultures incubated in the absence of lactoperoxidase, after incubation for 24 h. The urease activity and viability of H. pylori were both inactivated by this system in phosphate buffer. These effects were dependent on the concentrations of both lactoperoxidase and hydrogen peroxide and were abolished by the addition of cysteine. Furthermore, these effects were observed when bovine lactoperoxidase was replaced by recombinant human lactoperoxidase or native or recombinant human myeloperoxidase. The peroxidase system found in saliva and milk may contribute to the host defence against H. pylori infection and inhibition of transmission via the oral route.
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PMID:Susceptibility of Helicobacter pylori and its urease activity to the peroxidase-hydrogen peroxide-thiocyanate antimicrobial system. 1187 18

A new Gram-positive, nonpigmented, rod-shaped fluoride-tolerant bacterial strain, NM25, was isolated from waterlogged muddy field soil collected from the fluoride endemic area of Rampurhat II block (average fluoride in water, 4.7 mg/l, and in soil, 1.5 mg/kg) in Birbhum District, West Bengal, India. The study was undertaken to characterize the fluoride-tolerant bacterial isolate, to determine its role in bioaccumulation of fluoride, and to analyze the water and soil quality of the bacterial environment. The isolate was positive for catalase, lipase, urease, protease, oxidase, and H2S production, but negative for indole production, nitrate reduction, and Vogues-Proskauer test. The organisms were sensitive to recommended doses of ofloxacin, kanamycin, rifampicin, levofloxacin, vancomycin, gatifloxacin, gentamicin, doxycycline, streptomycin, and nalidixic acid but resistant to ampicillin. Based on the phenotypic characteristics, 16S rRNA gene sequence, and phylogenetic analysis, the bacterial isolate NM25 was identified as Bacillus flexus. The G+C content of the 16S rDNA was 53.14 mol%. This strain tolerated up to 20% (w/v) NaCl in nutrient agar medium and was grown at the pH range 4-12. It reduced fluoride concentration up to 67.45% and tolerated more than 1,500 ppm of fluoride in brain-heart infusion agar medium.
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PMID:Characterization of fluoride-tolerant halophilic Bacillus flexus NM25 (HQ875778) isolated from fluoride-affected soil in Birbhum District, West Bengal, India. 2406 84

The objective of this study was to create a biphasic cultural method for the detection of Helicobacter pylori in gastric biopsy specimens. The biphasic systems were made by using a urea agar slant with overlaying broth in a single vessel. Initially, three different liquid media including brain-heart infusion broth, Brucella broth, and Bolton broth were tested for their ability to support the growth of H. pylori. Bolton broth with 10% defibrinated horse blood demonstrated a significant increase in the numbers of H. pylori (p<0.05). The result showed that positive urease was used to concentrate viable H. pylori cells where the numbers of bacteria were 10(5)cfu. In addition, the reliable incubation time was at least 36h. In total, 55 biopsies were comparatively studied using commercial rapid urease test and PCR. Seven samples (12.72%) were positive with H. pylori by the biphasic test. With the CLOtest, 6 (10.91%) samples were positive. In conclusion, the Hp biphasic test achieved more positive samples than did the commercial rapid urease test.
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PMID:A new biphasic test for the detection of Helicobacter pylori in gastric biopsies. 2420 Jul 9