Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:6.3.4.6 (
urease
)
7,490
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Manuka honey (Leptospermum scoparium) exerts a strong antibacterial effect. Bacterial enzymes are an important target for antibacterial compounds. The enzyme
urease
produces ammonia and enables bacteria to adapt to an acidic environment. A new enzymatic assay, based on photometric detection of ammonia with ninhydrin, was developed to study
urease
activity.
Methylglyoxal
(MGO) and its precursor dihydroxyacetone (DHA), which are naturally present in manuka honey, were identified as jack bean
urease
inhibitors with IC
50
values of 2.8 and 5.0mM, respectively. Urease inhibition of manuka honey correlates with its MGO and DHA content. Non-manuka honeys, which lack MGO and DHA, showed significantly less
urease
inhibition. MGO depletion from manuka honey with glyoxalase reduced
urease
inhibition. Therefore,
urease
inhibition by manuka honey is mainly due to MGO and DHA. The results obtained with jack bean
urease
as a model
urease
, may contribute to the understanding of bacterial inhibition by manuka honey.
...
PMID:Manuka honey (Leptospermum scoparium) inhibits jack bean urease activity due to methylglyoxal and dihydroxyacetone. 2840 46
