Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:6.3.4.6 (urease)
 7,490 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The effect of LC(50) (1.8 MG/L) and a sublethal concentration (0.03 mg/L) of in vivo mercuric chloride exposure on the activities of acid and alkaline phosphatases, glucose-6-phosphatase, lipase and urease in the kidneys and ovaries of a teleost fish, Channa punctatus has been studied after 96 hr and 30 days respectively. It has been observed that the activities of all the enzymes except acid phosphatase were significantly inhibited in both the tissues. However, treatment for 96 hr resulted in more marked inhibition than 30 days of treatment. Acid phosphatase showed elevation in activity in the kidney after 96 hr of treatment.
 ...
PMID:Mercuric chloride induced enzymological changes in kidney and ovary of a teleost fish, Channa punctatus. 22 98

The effect of LC(50) and a sublethal concentration of lead nitrate on the activities of alkaline phosphatase, acid phosphatase, glucose-6-phosphatase, lipase and urease in the kidneys and ovaries of a teleost fish, Channa punctatus has been examined after 96 hr and 30 days respectively. The results show that all the five enzymes in the two tissues are inhibited significantly at both the experimental stages. However, the inhibition produced after 30 days by the sublethal concentration ish higher indicating the cumulative action of lead. Further, the inhibition of enzymes is, more marked in kidney than in the ovary.
 ...
PMID:Effects of lead nitrate on the activities of a few enzymes in the kidney and ovary Heteropneustes fossillis. 22

More than 100 strains of Corynebacterium genitalium, probably responsible for coryneform urethritis and other infections, and 600 commensals of the male and female urogenital tracts have been studied and grouped into five pathogenic types numbered I to V and six saprophytic types designated C-1 to C-6 on the basis of eight biological reactions. This preliminary classification has been based on differences in requirements for oxygen, on the fermentation of fructose, dextrose, sucrose, and starch together with the production of the enzymes gelatinase, lipase, and urease. One criterion differentiated the pathogens from the commensals: All pathogens were nonfructose fermenters whereas every commensal fermented this sugar.
 ...
PMID:A diagnostic key employing biological reactions for differentiating pathogenic Corynebacterium genitalium (NSU corynebacteria) from commensals of the urogenital tract. 35 42

The enzymes used in the identification of Gram negative bacteria belonging to the families of Enterobacteriaceae, Vibrionaceae, Parvobacteriaceae, Pseudomonadaceae and to the genera Alteromonas, Xanthomonas, Alkaligenes, Flavobacterium are classified arbitrarily by the author into enzymes essential for the diagnosis of the family (oxidase, nitratase), enzymes useful in the diagnosis of the genus or the species (ONPG-hydrolase, urease, oxidative desaminase, lysine decarboxylase and ornithine, arginine dihydrolase, thiosulphate reductase, pectinase), and into enzymes sought to confirm the diagnosis (tetrathionate reductase, gelatinase, lipase, DNase, amylase, beta-xylosidase, lecithinase). The technics permitting their identification are described and their distribution in the species and genera studied is reported.
 ...
PMID:[Research technics of enzymes used in the diagnosis of gram negative bacteria (author's transl)]. 74 48

Selected strains of methicillin- and aminoglycoside-resistant Staphylococcus aureus (MARSA) were subjected to a preliminary examination. They were representative of a larger group collected in a routine clinical microbiology laboratory over a period of 2 years. MARSA was endemic in the associated hospital. The characteristics investigated were antimicrobial resistance, the production of beta-lactamase, free and bound coagulase, protein A, DNA-ase, urease, lipase and pigment. The MARSA strains were generally indistinguishable, other than in their antimicrobial resistances. The resistance to methicillin was completely homogeneous. Except with imipenem, growth extended to the edge of discs containing methicillin and the other beta-lactam antibiotics tested when the strains were cultured at 37 degrees C on media without added salt. Homogeneous resistance may confer an epidemiological advantage on strains of this phenotype.
 ...
PMID:Partial characterization of an endemic strain of a methicillin- and aminoglycoside-resistant Staphylococcus aureus (MARSA) homogeneously resistant to beta-lactam antibiotics. 135 87

Using a protein isolated from soy, a dynamic water adsorption method was developed and the data were compared with those obtained from a static gravimetric procedure. Both methods gave comparable results, showing that Type II isotherms with considerable hysteresis were obtained. However, the dynamic procedure was preferred since it provided data rapidly and used significantly less material. Using the dynamic method, water adsorption isotherms at 25 degrees C were also determined for four biologically active proteins: alpha-amylase, beta-glucuronidase, lipase, and urease. BET (Brunauer, Emmet, and Teller) parameters were calculated and the specific surface areas for the native, biologically active proteins were found to be similar, 238.4 +/- 20.2 m2/g. On the other hand, the specific surface area for the denatured soy protein isolate was 144.6 m2/g. Nevertheless, the heat of absorbance for all of the proteins examined was similar, suggesting that they have comparable degrees of hydrophilicity.
 ...
PMID:Water vapor adsorption and desorption isotherms of biologically active proteins. 202 66

It was found that strains of T. equinum which were tested, produced in vitro urease, gelatinase, protease for bovine serum, hemolysins for horse and sheep red blood cells, and keratinase for horse, bovine and canine hair. The ability to produce elastase and caseinase was not recorded. In urease test, it was noticed that after three passages on media with urea the time of appearance of positive reactions was shortened and all strains of T. equinum reacted positively during three days. In these studies some differences in enzyme production between T. equinum and T. mentagrophytes were recorded. Generally, T. mentagrophytes revealed stronger enzymatic properties because it produced the enzymes in two ranges of temperature, i.e. 26-28 degrees C and 37 degrees C, and in addition it dissolved horse sera. The lipase production by T. equinum in optimal temperature of growth 26-28 degrees C only, may be used as an additional test for differentiation between T. equinum and T. mentagrophytes. In penetration test, it was found that strains of T. equinum which were tested perforate in vitro horse, bovine and canine hairs but they do not attack human hair. Some differences were seen concerning the way of penetration of horse hair by the fungi. T. equinum produces rectangular and coaxial perforation organs but T. mentagrophytes produces coaxial ones only.
 ...
PMID:[Biochemical properties of Polish and standard strains of Trichophyton equinum]. 256 78

One hundred and forty seven isolates of Serratia marcescens were collected from diverse clinical and environmental sources in south-east Texas. Natural isolates were compared with hospital strains for the occurrence of 12 potential virulence determinants. Their overall frequency was as follows: haemolytic activity 48%; lecithinase 95%; lipase 95%; motility 99%; pigmentation 24%; plasmid carriage 46%; proteolytic activity 98%; siderophore activity 99%; urease activity 5%; mannose-sensitive haemagglutination 96%; mannose-resistant haemagglutination 61%; and mannose-resistant type-K haemagglutination (MR/K-HA) 68%. Clinical strains demonstrated a significantly higher occurrence of MR/K-HA (p less than 0.001) and non-pigmentation (p less than 0.01) than environmental isolates.
 ...
PMID:A survey of potential virulence factors in clinical and environmental isolates of Serratia marcescens. 352 99

Benzoyl- and isopentenoyl phosphoric triamides (BPA and IPA) strongly inhibited urease activities from jack bean, soybean, watermelon seed, Proteus mirabilis, P. rettgeri, P. vulgaris, Mycobacterium smegmatis, and Ureaplasma urealyticum. Their I50 values (the final concentration causing 50% inhibition), independent of enzyme source, were 2-21 nM, which are about 1,000-fold lower than that of caprylohydroxamic acid, one of the most potent urease inhibitors. ATP-urea amidolyase activity was inhibited 50% by BPA at a higher concentration of 0.28 mM, but was not affected by IPA even at 1.3 mM. Thirteen kinds of hydrolases (trypsin, chymotrypsin, thermolysin, leucine aminopeptidase, papain, lipase, alpha-amylase, glucuronidase, asparaginase, arylsulfatase, alkaline phosphatase, acid phosphatase, and true cholinesterase), two oxidoreductases (catalase and alcohol dehydrogenase), three transferases (glutamic-oxaloacetic aminotransferase, gamma-glutamyl transpeptidase, and arylsulfotransferase) and two kinases (pyruvate kinase and creatine kinase) were not affected at all even at 1 mM BPA and IPA. Exceptionally, pseudo-cholinesterase from human serum was inhibited by BPA and IPA, whose I50 values were 70 nM and 10 muM, respectively, using acetylthiocholine as a substrate. These values increased to 0.55 muM and 54 muM, respectively, when acetylcholine was used as a substrate. These results show that N-acylphosphoric triamides potently and specifically inhibit urease activity at concentrations of nM order.
 ...
PMID:Specific inhibition of urease by N-acylphosphoric triamides. 384 42

Activities of twelve hydrolytic enzymes in the digestive tract of young rabbits before weaning (4 weeks old) and adult rabbits (3 months old) were measured. The principal digestive enzymes in both groups of rabbits appeared to be amylase (EC 3.2.1.1), maltase (EC 3.2.1.20), pectinase (EC 3.2.1.15) and proteinases. The stomach of young rabbits contained most of the lipolytic activity and 45.7% of the total proteolytic activity of the digestive tract. The highest specific activities (per g digesta) of amylase, maltase and proteinase in young rabbits were found in the small intestine. Total activities (per segment) of amylase and maltase in the small intestine and the caecum were similar. Activities of cellulase (EC 3.2.1.4), inulinase (EC 3.2.1.7) and beta-glucosidase (EC 3.2.1.21) were low and activity of pectinase was fairly high in all segments of the digestive tract. The highest activity of urease (EC 3.5.1.5) was found in the caecum. Enzymic profiles of the colonic chymus resembled those of the caecum. Total hydrolytic activity was lower in the colon than in the caecum. Specific activities of amylase and invertase (EC 3.2.1.26) were lower and those of inulinase and lactase (EC 3.2.1.23) higher in 4-week-old rabbits than in 3-month-old rabbits. Gastric proteinase represented almost half of the total proteolytic activity of the digestive tract, whereas lipolytic activity of gastric contents was not found in measurable quantities in adult rabbits. The caecal contents of adult rabbits contained most of the total activity of lipase (EC 3.1.1.3), cellulase, xylanase (EC 3.2.1.32), pectinase, lactase, invertase, beta-glucosidase and urease present in the digestive tract.(ABSTRACT TRUNCATED AT 250 WORDS)
 ...
PMID:Distribution of activity of hydrolytic enzymes in the digestive tract of rabbits. 753 89


1 2 3 4 5 6 7 8 9 Next >>