Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:6.3.4.6 (
urease
)
7,490
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Pore-expanded
MCM
-41 (PE-MCM-41) silica exhibits a unique combination of high specific surface area (ca. 1000 m(2)/g), pore size (up to 25 nm) and pore volume (up to 3.5 cm(3)/g). As such, this material is highly suitable for the adsorption of large biomolecules. The current study focused primarily on the application of PE-
MCM
-41 material as suitable host for
urease
(nickel-based large metalloenzyme) in controlled hydrolysis of urea. Urease adsorbed on PE-
MCM
-41, regular
MCM
-41 and silica gel (SGA) were used as catalysts for urea hydrolysis reaction. Adsorption studies of
urease
on these materials from aqueous solution at pH 7.2 revealed that the adsorption capacity of PE-
MCM
-41 (102 mg/g) is significantly higher than that of
MCM
-41 (56 mg/g) and SGA (21 mg/g). The equilibrium adsorption data were well fitted using the Langmuir-Freundlich model. Furthermore, the kinetic study revealed that the uptake of
urease
follow the pseudo-first order kinetics. The in vitro urea hydrolysis reaction on pristine
urease
and different
urease
-loaded catalysts showed that the rate of hydrolysis reaction is significantly slower on U/PE-
MCM
-41 compared to that of bulk
urease
and
urease
on
MCM
-41 and SGA. This technique could be an alternative means to the use of
urease
inhibitors to control the ammonia release from urea fertilizer.
...
PMID:Adsorption of urease on PE-MCM-41 and its catalytic effect on hydrolysis of urea. 1796 95
