Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound   Target Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound

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Query: EC:6.3.4.6 (urease)
7,490 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

96 gastric mucosal biopsy specimens collected at endoscopy and placed into 3 ml 20% glucose were examined histologically, microscopically (Gram staining), by urease test, and by cultivation. The rate of positive findings ws 62.5% by urease test, 34.4% by cultivation, and 27.0% microscopically. By using simple selective solid media, the number of positive cultivations increased to 58.7%. Positive microbiological findings were established by one of three microbiological methods at a high percentage in gastric ulcers (87.5%) and at the lowest rate in duodenal ulcers (57.1%). Remarkable were the positive findings in 60% of patients with normal endoscopic results. In chronic gastritis the highest positivity rate was established histologically (52.6%). Antibiotics without bismuth preparations were used in the treatment of six patients. Check-up examinations yielded negative histological and microbiological findings only in two patients. We failed to affect the inflammatory activity of the gastric mucosa even when consolidation of the ulcer lesion was achieved.
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PMID:[Rapid detection and easy culture of Campylobacter pylori from human gastric mucosa]. 265 26

The presence of C pylori infection was determined in 1445 patients undergoing upper gastrointestinal endoscopy over a 12 month period. The presence of C pylori was detected in gastric mucosal biopsy specimens by the biopsy urease test, microscopy (Gram stained smears and histology) and culture. Two media were used for the biopsy urease test: Christensen's urea broth (for the first 600 patients) and the Christensen's urea broth modified by increasing the concentration of phenol red and omitting the nutrients, glucose and peptone (for the remaining patients). Both the Christensen's urea broth and modified urea broth were almost 100% specific when compared with detection of C pylori by Gram, culture and histopathology. The modified broth was more sensitive (96% sensitivity compared with culture) than the Christensen's broth (92% sensitivity) but this difference was not statistically significant. The modified broth gave significantly more positive results (58%) in less than 30 minutes than the Christensen's broth (48%). Seventy four per cent of positive results were available in less than two hours. Specimens from patients with extensive C pylori infection gave more rapid results: 86% of specimens that yielded a profuse growth of C pylori and 76% that contained numerous organisms on histological sections had a positive urease test in less than one hour. There was no significant difference between the specificity and sensitivity of our modified urea broth and the other modified broths described in the literature. This test is a cheap and rapid alternative to the diagnosis of C pylori by Gram stained smears or culture.
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PMID:Detection of Campylobacter pylori by the biopsy urease test: an assessment in 1445 patients. 276 1

In November 1985, a 5-year-old Chinese girl presented to the Dermatological Department of Chang Gung Memorial Hospital, Taipei, having scaly erythematous plaque with mild itching on her right upper eyelid. Skin biopsy and fungal cultures were performed after failure of initial topical steroid therapy. The histopathology revealed many acute and chronic inflammatory cells infiltrating the dermis and H & E stain revealed some foamy vacuolated spores; P.A.S. and Gomori's methenamine stain also showed many spores and sporangia containing endospores. Lactophenol cotton blue and methylene blue wet mount preparations were made from the colony growing on Sabouraud's agar. Microscopically, these showed many round or oval spores and endospore-containing sporangia, corresponding with the histopathology. This microorganism grew as a milky white yeast-like colony on Sabouraud's dextrose agar, blood agar, EMB, Tween 80 cornmeal agar, chocolate agar, MacConkey agar and brain heart infusion with sheep RBC agar. On Pagano-Levin medium, the colony became deep red in color and in the thioglycollate broth tube culture, it was suspended on the upper layer as a whitish ring-form of granules. The microorganism showed no urease activity. In the assimilation tests, there were positive reactions to glucose, galactose, trehalose, fructose, mannose and glycerol, and negative reactions to maltose, xylose, raffinose, sucrose, lactose, cellabiose, n-propanol, etc. The electronmicroscopic examination of the colony revealed sporangium containing spores and characteristic dense body and plastids in the spores.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:[Cutaneous protothecosis: first case in Taiwan]. 280 69

Six conventional biochemical tests were combined to produce an identification scheme. These tests included decarboxylation of lysine and ornithine, fermentation of glucose and cellobiose, indole production and urease production. Three hundred antibiotic resistant coliforms from urine specimens were tested by this scheme and also by the API 20E for comparison. Two hundred and seventy nine (93%) of organisms were correctly identified using the six tests, 17 (5.7%) were referred for further study, and four (1.3%) were misidentified. It is concluded that this combination of tests provides an inexpensive, accurate, and rapid tool for identification.
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PMID:Rapid conventional scheme for biochemical identification of antibiotic resistant enterobacteriaceae isolates from urine. 305 81

The biochemical characteristics of 114 respiratory Haemophilus isolates were examined by the Minitek and Microbact systems. The Microbact system was easy to use and read, although some of the less important reactions (glucose and xylose) were difficult to interpret on occasions. On the basis of the 3 crucial reactions--indole production, ornithine decarboxylase and urease activity--discrepancies between the two systems were minor. Given careful standardization of techniques the Microbact system is a suitable alternative to established techniques for the biotyping of H. influenzae and H. parainfluenzae.
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PMID:Biotyping respiratory Haemophilus species with the microbact system. 306 Aug 21

Commercial identification kits (API 20C, API Yeast-Ident and API-Zym) were compared with a conventional but simplified identification method (SIM) for identifying seventy-two yeast isolates from fresh sweet corn. SIM failed to provide identification of two isolates. Of the twenty species identified, only eleven were included in the API 20C profile index. Three isolates were identified at the species level and three were identified at the genus level with 100% accuracy. The enzyme kit (Yeast-Ident) gave rather unreliable results, in that identification of only four isolates with 75 to 85% of appropriate reactions was made. The API 20C kit could be used to identify non-clinical yeasts, provided they were included in its database and a few additional tests (urease reaction, nitrate assimilation and glucose fermentation) were also performed.
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PMID:Evaluation of simplified and commercial systems for identification of foodborne yeasts. 307 71

Numerical taxonomy procedures were used to study 118 strains of Bacillus isolated from non-sterile drugs prepared for oral administration. Similarities between pairs of strains were calculated by the simple matching coefficient of Sokal and Michener (SSM). Each strain was tested for 60 unit characters and three clusters were defined. The strains in each cluster presented a similarity level of at least 60%. Cluster A comprised the strains identified as Bacillus cereus (SSM = 93.13%), cluster B contained three subgroups corresponding to the species B. pumilus, B. subtilis and B. licheniformis (SSM = 84.35%) and cluster C also included three subgroups that belonged to the species B. firmus, B. lentus and B. badius (SSM = 80.14%). The most discriminating tests were selected to differentiate the clusters from the subgroups. The feature with the highest discriminating power between clusters A and B was the lack of acid production from arabinose and mannitol. The Voges-Proskauer, methyl red tests and sensitivity to polymyxin B clearly distinguished cluster A from C. The Voges-Proskauer test and acid production from arabinose were the best to differentiate between B and C. Bacillus pumilus and B. subtilis differed in starch hydrolysis and B. licheniformis in growing anaerobically. To discriminate B. firmus from B. lentus the most important tests were the acid production from glucose and sucrose; intermediate strains were found. Bacillus badius was differentiated from B. firmus by 10 tests, and from B. lentus by the production of urease.
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PMID:Numerical taxonomy of Bacillus isolated from orally administered drugs. 309 33

Cryptococcus neoformans var. neoformans (74 isolates) and C. neoformans var. gattii (44 isolates) were used to test urease activity after growth on both yeast extract-glucose-peptone agar (YEPG) and on YEPG supplemented with 100 microM EDTA. Every isolate grown on YEPG agar for 48 h at 30 degrees C produced a positive reaction within 1 h in a modified rapid urease assay at 37 degrees C. However, isolates grown on YEPG with 100 microM EDTA showed a distinct pattern which corresponded to their varietal status. All but 1 of 74 C. neoformans var. neoformans isolates (98.7%) produced a positive reaction within 1 to 4 h, while none of 44 C. neoformans var. gattii isolates produced a positive reaction within the same period. The urease inhibition results and the canavanine-glycine-bromthymol blue agar test results showed 100% correlation among isolates of C. neoformans var. gattii and 98.7% correlation among isolates of C. neoformans var. neoformans. Two representative isolates of C. neoformans var. gattii (serotypes B and C) were further tested for urease during a prolonged incubation period in urea broth. These isolates failed to show a positive reaction even after 11 h of incubation. The uptake of EDTA was negligible in the two varieties. Extracts of cells grown on YEPA agar showed a high level of urease activity in both varieties. Extracts of cells grown on the agar with 100 microM EDTA showed a marked reduction (86%) of urease activity in one isolate of C. neoformans var. gattii but showed only a 30% reduction in one isolate of C. neoformans var. neoformans. Based on these results, the differential effect of EDTA on the two varieties of C. neoformans appeared to be due to greater inhibition of urease synthesis in C. neoformans var. gattii.
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PMID:Urease inhibition by EDTA in the two varieties of Cryptococcus neoformans. 311 9

The comparative study of 44 isolates of Corynebacterium group D2, from urine, most frequently, shows the pathogenic role of these bacteria in urinary tract infection, with or without urinary stones. These microorganisms have an opportunistic behaviour in other non-urinary sites, and become pathogen in immunosuppressed conditions. The rapid tests as urease, glucose acidification, nitrate reductase, associated with multiple resistance to antibiotics (beta-lactams and aminosides) identify easily Corynebacterium group D2, from 48 h cultures under CO2 conditions. The results of MIC determination of 10 antibiotics, show the high activity (100% sensitivity) of vancomycin and pristinamycin, with MIC modes, respectively, 0.5 and 0.03 mg/l. These antibiotics are the most useful for the treatment of non-urinary infections. Among quinolones, the most active agents are ciprofloxacin and ofloxacin (MIC modes: 4 and 2 mg/l), so these antimicrobials could be used for the treatment of urinary tract infections caused by Corynebacterium group D2.
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PMID:[Corynebacterium group D2. Clinical study, biochemical identification and antibiotic sensitivity]. 313 26

The biochemical properties of 39 strains of Haemophilus avium from chickens were determined. All the strains produced acid from fructose, galactose, glucose and mannose but not from lactose. Variable reactions were found for arabinose, maltose, mannitol, sorbitol, trehalose and xylose. No strains showed urease activity or produced indole, while beta-galactosidase and/or ornithine decarboxylase activity was present in some strains. This variability allowed the recognition of 15 biochemical biovars including some not previously recognized in H. avium. Only 25 (64%) of the H. avium strains could be assigned to the three species (Pasteurella avium, P. volantium and Pasteurella species A) recently proposed to replace H. avium.
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PMID:Biochemical properties of catalase-positive avian haemophili. 315 Dec 6


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