Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:6.3.4.6 (
urease
)
7,490
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Isolates (n = 94) of Corynebacterium pseudotuberculosis were obtained from sheep, goats, horses, and cattle from various parts of the world. The isolates were characterized biochemically and by restriction endonuclease analysis of DNA. We found near homogeneity in the ability of isolates to ferment carbohydrates and to produce
urease
. All isolates produced
phospholipase D
and catalase. The ability of isolates from horses to reduce nitrate, the inability of isolates from sheep and goats to do so, and the correlation of this characteristic with results of restriction endonuclease analyses confirmed the existence of 2 biovars of C pseudotuberculosis. We propose that these biovars be referred to as biovar equi for isolates that reduce nitrate and biovar ovis for isolates that fail to do so.
...
PMID:Biochemical and genetic characterization of Corynebacterium pseudotuberculosis. 283 63
Twenty-five strains of Corynebacterium pseudotuberculosis isolated from lesions of caseous lymphadenitis in goats were examined for their biochemical characteristics, antimicrobial susceptibility, and
phospholipase D
activity. The strains were uniform in biochemical reactions, cultural characteristics, and susceptibility to antimicrobial agents. Presence of
urease
and
phospholipase D
and absence of pyrazinamidase were valuable criteria in the identification of C. pseudotuberculosis.
...
PMID:Characterization of strains of corynebacterium pseudotuberculosis. 709 13
A total of 34
Corynebacterium
sp. strains were isolated from caseous lymph node abscesses of wild boar and roe deer in different regions of Germany. They showed slow growth on Columbia sheep blood agar and sparse growth on Hoyle's tellurite agar. Cellular fatty acid analysis allocated them in the
C. diphtheriae
group of genus
Corynebacterium
. MALDI-TOF MS using specific database extensions and
rpoB
sequencing resulted in classification as
C. ulcerans
. Their quinone system is similar to
C. ulcerans
, with major menaquinone MK-8(H2). Their complex polar lipid profile includes major lipids phosphatidylinositol, phosphatidylinositol-mannoside, diphosphatidylglycerol, but also unidentified glycolipids, distinguishing them clearly from
C. ulcerans
. They ferment glucose, ribose and maltose (like
C. ulcerans
), but do not utilise d-xylose, mannitol, lactose, sucrose and glycogen (like
C. pseudotuberculosis
). They showed activity of catalase,
urease
and
phospholipase D
, but variable results for alkaline phosphatase and alpha-glucosidase. All were non-toxigenic,
tox
gene bearing and susceptible to clindamycin, penicillin and erythromycin. In 16SrRNA gene and RpoB protein phylogenies the strains formed distinct brancheswith
C. ulcerans
as nearest relative.Whole genome sequencing revealed the unique sequence type 578, a distinctbranch in pangenomic core genome MLST, average nucleotide identities <91%, enhancedgenome sizes (2.55 Mbp) and G/C content (54.4 mol%) compared to related species.These results suggest that the strains represent a novel species, for which wepropose the name
Corynebactriumsilvaticum
sp. nov., based on their first isolation from forest-dwellinggame animals. The type strain isKL0182
T
(= CVUAS 4292
T
= DSM 109166
T
= LMG 31313
T
= CIP 111 672
T
).
...
PMID:
Corynebacterium silvaticum
sp. nov., a unique group of NTTB corynebacteria in wild boar and roe deer. 3236 99
