Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:6.3.4.6 (
urease
)
7,490
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Helicobacter pylori
, a gastroenteric pathogen believed to have co-evolved with humans over 100,000 years, shows significant genetic variability. This motivates the study of different
H. pylori
strains and the diseases they cause in order to identify determinants for disease evolution. In this study, we used proteomics tools to compare two
H. pylori
strains. Nic25_A was isolated in Nicaragua from a patient with intestinal metaplasia, and
P12
was isolated in Europe from a patient with duodenal ulcers. Differences in the abundance of surface proteins between the two strains were determined with two mass spectrometry-based methods, label-free quantification (MaxQuant) or the use of tandem mass tags (TMT). Each approach used a lipid-based protein immobilization (LPI
TM
) technique to enrich peptides of surface proteins. Using the MaxQuant software, we found 52 proteins that differed significantly in abundance between the two strains (up- or downregulated by a factor of 1.5); with TMT, we found 18 proteins that differed in abundance between the strains. Strain
P12
had a higher abundance of proteins encoded by the
cag
pathogenicity island, while levels of the acid response regulator ArsR and its regulatory targets (KatA, AmiE, and proteins involved in
urease
production) were higher in strain Nic25_A. Our results show that differences in protein abundance between
H. pylori
strains can be detected with proteomic approaches; this could have important implications for the study of disease progression.
...
PMID:Comparative Analysis of Two
Helicobacter pylori
Strains using Genomics and Mass Spectrometry-Based Proteomics. 2789 Nov 14
