Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:6.3.4.6 (
urease
)
7,490
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Culture-independent studies have identified DNA of bacterial pathogens in the gallbladder under pathological conditions, yet reports on the isolation of corresponding live bacteria are rare. Thus, it is unclear which pathogens, or pathogen communities, can colonize the gallbladder and cause disease. Using light microscopy, scanning electron microscopy, culture techniques, phylogenetic analysis,
urease
assays and Western blotting, we investigated the presence of live bacterial communities in the gallbladder of a cholecystitis patient after cholecystectomy. 16S rRNA gene sequencing of isolated bacterial colonies revealed the presence of pathogens most closely resembling
Corynebacterium urinapleomorphum
nov. sp.,
Staphylococcus saprophyticus
and
Helicobacter pylori
. The latter colonies were confirmed as
H. pylori
by immunohistochemistry and biochemical methods.
H. pylori
cultured from the gallbladder exhibited both the same DNA fingerprinting and Western
cagA
gene sequence with ABC-type EPIYA (Glu-Pro-Ile-Tyr-Ala) phosphorylation motifs as isolates recovered from the gastric mucus of the same patient, suggesting that gastric
H. pylori
can also colonize other organs in the human body. Taken together, here we report, for the first time, the identification and characterization of a community consisting of live
S. saprophyticus
;
C. urinapleomorphum
, and
H. pylori
in the gallbladder of a patient with
acute cholecystitis
. Their potential infection routes and roles in pathogenesis are discussed.
...
PMID:Unusual Manifestation of Live
Staphylococcus saprophyticus, Corynebacterium urinapleomorphum
, and
Helicobacter pylori
in the Gallbladder with Cholecystitis. 2993 76
