Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:6.3.4.6 (urease)
 7,490 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Antibodies to tetanus toxin were induced in sheep by hyperimmunization over 24 weeks. Bleeds taken at weeks 4, 8, 20 and 30 were assayed for antibody titre by both an enzyme immunoassay (EIA) using a newly-described urease enzyme/substrate system and by bioassay in mice. There was a very good correlation between the two assay systems and, with the exception of the week 4 Bleeds, the relationship was the same at all stages of hyperimmunization regardless of titre, adjuvant, or whether toxin or toxoid was used as immunogen or for coating the plates. The results establish that the EIA can replace the bioassay for the determination of tetanus antitoxin in ovine sera.
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PMID:A comparison of enzyme immunoassay and bioassay for the quantitative determination of antibodies to tetanus toxin. 634 47

The outer surface protein A (OspA) lipoprotein of Borrelia burgdorferi, like cholera toxin and the heat-labile enterotoxin of Escherichia coli, induces pro-inflammatory cytokines. This suggested that, like those toxins, OspA might be a mucosal immunogen and adjuvant. OspA, administered intranasally (i.n.) or intragastrically, induced strong serum IgG and salivary gland IgA responses. The serum IgG isotypes were indicative of a mixed T helper 1 and T helper 2 response, the latter being more pronounced. The N-terminal tripalmitoyl-S-glyceryl-cysteine (Pam3Cys) lipid moiety was absolutely required. OspA strongly enhanced the serum IgG and salivary gland IgA responses to jack bean urease co-administered by the i.n. route. OspA also enhanced the response to tetanus toxoid and induced limited protection against challenge. A synthetic lipopeptide also adjuvanted the response to urease by the i.n. route, but was ca 500-fold less potent on a molar basis than OspA. These results suggest that OspA or other lipoproteins may be useful in mucosal vaccines.
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PMID:OspA lipoprotein of Borrelia burgdorferi is a mucosal immunogen and adjuvant. 926 45