Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:6.3.2.3 (
glutathione synthetase
)
678
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Ubiquitous free radical production occurs continuously in cells and tissues. Glutathione is the most abundant mammalian antioxidant, and is synthesized by
glutathione synthetase
(
GSS
). Therefore,
GSS
plays an important role in defending the cell against reactive oxygen species. The expression of
GSS
has been studied in human cells; however, sequence information about alternative splicing variants of
GSS
mRNA has not been reported. In the present study, we identified a novel alternative splicing variant (ASV) of the
GSS
gene in 10 human normal tissues and five human cancer cell lines. The deleted transcript of
GSS
was characterized by an in-frame deletion of 333 bp, corresponding to the complete loss of exons 4 and 5. Thus this
GSS
ASV causes protein truncation. We quantified the mRNA of
GSS
ASV in human normal tissues using real-time PCR. The ASV was detected in colon, kidney, lung, liver, placenta, peripheral blood and
uterus
, but not in heart, skeletal muscle and spleen tissue. Our results provide a basis for more detailed studies on the regulation of
GSS
, and for further evaluation of this and other possible roles of
GSS
. Understanding the regulation of
GSS
expression is very important for the development of new strategies for controlling the development of GSH-based redox homeostasis.
...
PMID:Alternative RNA splicing in expression of the glutathione synthetase gene in human cells. 1967 93
