Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:6.3.2.19 (
ubiquitin-protein ligase
)
799
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Mutations and ectopic
FAT1
cadherin expression are implicated in a broad spectrum of diseases ranging from developmental disorders to cancer. The regulation of
FAT1
and its downstream signalling pathways remain incompletely understood. We hypothesized that identification of additional proteins interacting with the
FAT1
cytoplasmic tail would further delineate its regulation and function. A yeast two-hybrid library screen carried out against the juxtamembrane region of the cytoplasmic tail of
FAT1
identified the E3
ubiquitin-protein ligase
SH3RF1 as the most frequently recovered protein-binding partner. Ablating SH3RF1 using siRNA increased cellular
FAT1
protein levels and stabilized expression at the cell surface, while overexpression of SH3RF1 reduced
FAT1
levels. We conclude that SH3RF1 acts as a negative post-translational regulator of
FAT1
levels.
...
PMID:Protein interaction screening identifies SH3RF1 as a new regulator of FAT1 protein levels. 2812 44
