Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:6.3.2.19 (
ubiquitin-protein ligase
)
799
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The transcription factor
E2F-1
is important in the control of cell proliferation. Its activity must be tightly regulated in a cell-cycle-dependent manner to enable programs of gene expression to be coupled closely with cell-cycle position. Here we show that, following its accumulation in the late G1 phase of the cell cycle,
E2F-1
is rapidly degraded in S/G2 phase. This event is linked to a specific interaction of
E2F-1
with the F-box-containing protein p45SKP2, which is the cell-cycle-regulated component of the
ubiquitin-protein ligase
SCFSKP2 that recognizes substrates for this ligase. Disruption of the interaction between
E2F-1
and p45SKP2 results in a reduction in ubiquitination of
E2F-1
and the stabilization and accumulation of transcriptionally active
E2F-1
protein. These results indicate that an SCFSKP2-dependent ubiquitination pathway may be involved in the downregulation of
E2F-1
activity in the S/G2 phase of the cell cycle, and suggest a link between SCFSKP2 and cell-cycle-dependent gene control.
...
PMID:Interaction between ubiquitin-protein ligase SCFSKP2 and E2F-1 underlies the regulation of E2F-1 degradation. 1055 70
