Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:6.2.1.7 (
BAL
)
1,977
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
We measured transiently-expressed beta-galactosidase activity by introducing the mouse
myelin basic protein
(
MBP
)-lacZ chimeric gene (
MBP
-lacZ) into the NG108-15 neuronal/glial hybrid cell line. Deletion studies of the promoter region of the
MBP
gene showed that the promoter region between -1318 bp and -254 bp might contain sequences that repress
MBP
promoter activity. Fine deletion analysis using
BAL
31 exonuclease revealed sequences between bp -208 and -140, -139 and -118, and -89 and -75 which were critical for promoter activity in NG 108-15 cells. DNaseI footprinting analysis revealed a cellular factor(s) that bind to the promoter region between bp -127 and -106 with NG108-15 whole cell extracts. The SV40 promoter was activated by insertion of the sequences around the region protected in footprinting experiments, in a manner independent of its orientation in NG108-15 cells. This protected region is thought to be one of the critical cis-acting DNA elements for efficient transcription.
...
PMID:The promoter elements of the mouse myelin basic protein gene function efficiently in NG108-15 neuronal/glial cells. 247 Jun 51
